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GRP78 Kit ELISA

HSPA5 Reactivité: Humain Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5654966
  • Antigène Voir toutes GRP78 (HSPA5) Kits ELISA
    GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))
    Reactivité
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.312 ng/mL - 20 ng/mL
    Seuil minimal de détection
    0.312 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Heat Shock 70 kDa Protein 5 (HSPA5). No significant cross-reactivity or interference between Heat Shock 70 kDa Protein 5 (HSPA5) and analogues was observed.
    Sensibilité
    0.129 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70 kDa Protein 5 (HSPA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70 kDa Protein 5 (HSPA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70 kDa Protein 5 (HSPA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 70 kDa Protein 5 (HSPA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 (HSPA5) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock 70kDa Protein 5 (HSPA5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes GRP78 (HSPA5) Kits ELISA
    GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))
    Autre désignation
    Heat Shock 70kDa Protein 5 (HSPA5 Produits)
    Synonymes
    GRP78 Kit ELISA, BiP Kit ELISA, GRP-78 Kit ELISA, grp78 Kit ELISA, hspa5a Kit ELISA, BIP Kit ELISA, MIF2 Kit ELISA, AL022860 Kit ELISA, AU019543 Kit ELISA, Bip Kit ELISA, D2Wsu141e Kit ELISA, D2Wsu17e Kit ELISA, Grp78 Kit ELISA, Hsce70 Kit ELISA, SEZ-7 Kit ELISA, Sez7 Kit ELISA, baffled Kit ELISA, mBiP Kit ELISA, cb865 Kit ELISA, fb60h09 Kit ELISA, fi36d04 Kit ELISA, wu:fb60h09 Kit ELISA, wu:fi36d04 Kit ELISA, zgc:55994 Kit ELISA, zgc:77606 Kit ELISA, 78 kDa glucose-regulated protein Kit ELISA, heat shock protein family A (Hsp70) member 5 Kit ELISA, BiP/GRP78 Kit ELISA, glucose-regulated protein 78 Kit ELISA, putative glucose-regulated protein 78 Kit ELISA, Hsp70 family ATPase KAR2 Kit ELISA, heat shock protein family A (Hsp70) member 5 S homeolog Kit ELISA, heat shock 70 kDa protein 5a Kit ELISA, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) Kit ELISA, heat shock protein 5 Kit ELISA, heat shock protein family A member 5 Kit ELISA, CpipJ_CPIJ003550 Kit ELISA, HSPA5 Kit ELISA, grp78 Kit ELISA, LOC100533358 Kit ELISA, BiP/grp78 Kit ELISA, Tc00.1047053506585.40 Kit ELISA, Tb11.02.5450 Kit ELISA, Tb11.02.5500 Kit ELISA, LMJF_28_1200 Kit ELISA, KAR2 Kit ELISA, LOC100135840 Kit ELISA, hspa5.S Kit ELISA, hspa5 Kit ELISA, Hspa5 Kit ELISA
    Sujet

    Gene Name: Heat Shock 70 kDa Protein 5

    Gene Aliases: MIF2, BIP, GRP78, Immunoglobulin heavy chain-binding protein, Glucose Regulated Protein 78, Binding Immunoglobulin Protein, Endoplasmic reticulum lumenal Ca binding grp78

    Pathways
    Thyroid Hormone Synthesis, ER-Nucleus Signaling
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