Sonic Hedgehog Kit ELISA

N° du produit ABIN5655006

Détail du produit Sonic Hedgehog Kit ELISA

Antigène: Sonic Hedgehog (SHH)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Hedgehog Homolog, Sonic (SHH). No significant cross-reactivity or interference between Hedgehog Homolog, Sonic (SHH) and analogues was observed.

Sensibilité: 30 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hedgehog Homolog, Sonic (SHH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hedgehog Homolog, Sonic (SHH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hedgehog Homolog, Sonic (SHH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hedgehog Homolog, Sonic (SHH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hedgehog Homolog, Sonic (SHH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hedgehog Homolog, Sonic (SHH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Sonic Hedgehog (SHH)

Autre désignation: Hedgehog Homolog, Sonic (SHH Produits)

Synonymes: HHG1 Kit ELISA, HLP3 Kit ELISA, HPE3 Kit ELISA, MCOPCB5 Kit ELISA, SMMCI Kit ELISA, TPT Kit ELISA, TPTPS Kit ELISA, 9530036O11Rik Kit ELISA, Dsh Kit ELISA, Hhg1 Kit ELISA, Hx Kit ELISA, Hxl3 Kit ELISA, M100081 Kit ELISA, fc83d08 Kit ELISA, shh Kit ELISA, syu Kit ELISA, vhh-1 Kit ELISA, vhh1 Kit ELISA, wu:fc83d08 Kit ELISA, Xhh Kit ELISA, hedgehog Kit ELISA, xshh Kit ELISA, SHH Kit ELISA, twh Kit ELISA, twhh Kit ELISA, sonic hedgehog Kit ELISA, sonic hedgehog a Kit ELISA, sonic hedgehog L homeolog Kit ELISA, sonic hedgehog protein A Kit ELISA, sonic hedgehog b Kit ELISA, SHH Kit ELISA, Shh Kit ELISA, shha Kit ELISA, shh.L Kit ELISA, shh Kit ELISA, shhb Kit ELISA

Sujet:

Gene Name: Hedgehog Homolog, Sonic

Gene Aliases: HHG1, HLP3, HPE3, SMMCI, SHHN, SHH-N

ID gène: 6469

UniProt: Q15465

Pathways: Signalisation Hedgehog, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development