HS2ST1 Kit ELISA

N° du produit ABIN5655045

Détail du produit HS2ST1 Kit ELISA

Antigène: HS2ST1 (Heparan Sulfate 2-O-Sulfotransferase 1 (HS2ST1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1). No significant cross-reactivity or interference between Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1) and analogues was observed.

Sensibilité: 0.052 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: HS2ST1 (Heparan Sulfate 2-O-Sulfotransferase 1 (HS2ST1))

Autre désignation: Heparan Sulfate-2-O-Sulfotransferase 1 (HS2ST1 Produits)

Synonymes: HS2ST1 Kit ELISA, hs2st Kit ELISA, dJ604K5.2 Kit ELISA, AW214369 Kit ELISA, Hs2st Kit ELISA, mKIAA0448 Kit ELISA, CHS2ST Kit ELISA, HS2ST Kit ELISA, 2-OST Kit ELISA, 2OST Kit ELISA, HS2st Kit ELISA, hs2st1 Kit ELISA, im:7147474 Kit ELISA, zgc:158230 Kit ELISA, heparan sulfate 2-O-sulfotransferase 1 Kit ELISA, Heparan sulfate 2-O-sulfotransferase 1 Kit ELISA, heparan sulfate 2-O-sulfotransferase 1 L homeolog Kit ELISA, heparan sulfate 2-O-sulfotransferase 1a Kit ELISA, HS2ST1 Kit ELISA, Tsp_05909 Kit ELISA, hs2st1 Kit ELISA, hs2st Kit ELISA, Hs2st1 Kit ELISA, hs2st1.L Kit ELISA, hs2st1a Kit ELISA

Sujet:

Gene Name: Heparan Sulfate-2-O-Sulfotransferase 1

ID gène: 9653

UniProt: Q7LGA3

Pathways: Glycosaminoglycan Metabolic Process, Tube Formation, SARS-CoV-2 Protein Interactome