IGF2 Kit ELISA

N° du produit ABIN5655408

Détail du produit IGF2 Kit ELISA

Antigène: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.625 ng/mL - 40 ng/mL

Seuil minimal de détection: 0.625 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor 2 (IGF2). No significant cross-reactivity or interference between Insulin Like Growth Factor 2 (IGF2) and analogues was observed.

Sensibilité: 0.256 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 2 (IGF2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 2 (IGF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 2 (IGF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor 2 (IGF2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor 2 (IGF2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor 2 (IGF2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Autre désignation: Insulin Like Growth Factor 2 (IGF2 Produits)

Synonymes: C11orf43 Kit ELISA, IGF-II Kit ELISA, PP9974 Kit ELISA, LOC100136550 Kit ELISA, igf2 Kit ELISA, igf2b Kit ELISA, wu:fc26e03 Kit ELISA, zgc:91781 Kit ELISA, igf-2 Kit ELISA, insigf Kit ELISA, pp9974 Kit ELISA, AL033362 Kit ELISA, Igf-2 Kit ELISA, Igf-II Kit ELISA, M6pr Kit ELISA, Mpr Kit ELISA, Peg2 Kit ELISA, IGFII Kit ELISA, RNIGF2 Kit ELISA, IGF-II-B Kit ELISA, igf2-a Kit ELISA, igf2-b Kit ELISA, insulin like growth factor 2 Kit ELISA, insulin-like growth factor II Kit ELISA, insulin-like growth factor 2a Kit ELISA, insulin like growth factor 2 S homeolog Kit ELISA, insulin-like growth factor 2 Kit ELISA, insulin-like growth factor 2 (somatomedin A) Kit ELISA, insulin like growth factor 2 L homeolog Kit ELISA, IGF2 Kit ELISA, LOC100136550 Kit ELISA, igf2a Kit ELISA, igf2.S Kit ELISA, Igf2 Kit ELISA, igf2.L Kit ELISA

Sujet:

Gene Name: Insulin Like Growth Factor 2

Gene Aliases: INSIGF, IGF-II, Somatomedin A, Preptin, Insulin-like growth factor II Ala-25 Del

ID gène: 3481

UniProt: P01344

Pathways: Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation