IGFBPI Kit ELISA

N° du produit ABIN5655417

Détail du produit IGFBPI Kit ELISA

Antigène: IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

Reactivité: Boeuf (Vache)

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein 1 (IGFBP1). No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein 1 (IGFBP1) and analogues was observed.

Sensibilité: 0.31 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein 1 (IGFBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein 1 (IGFBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor Binding Protein 1 (IGFBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Like Growth Factor Binding Protein 1 (IGFBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 1 (IGFBP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 1 (IGFBP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

Autre désignation: Insulin Like Growth Factor Binding Protein 1 (IGFBPI Produits)

Synonymes: cb656 Kit ELISA, igfbp1 Kit ELISA, IGFBP-1 Kit ELISA, MGC68538 Kit ELISA, afbp Kit ELISA, ibp1 Kit ELISA, pp12 Kit ELISA, igf-bp25 Kit ELISA, higfbp-1 Kit ELISA, IGFBP1 Kit ELISA, LOC100223208 Kit ELISA, LOC100305121 Kit ELISA, AFBP Kit ELISA, IBP1 Kit ELISA, IGF-BP25 Kit ELISA, PP12 Kit ELISA, hIGFBP-1 Kit ELISA, IGFBA Kit ELISA, insulin like growth factor binding protein 1 Kit ELISA, insulin-like growth factor binding protein 1a Kit ELISA, insulin like growth factor binding protein 1 S homeolog Kit ELISA, insulin like growth factor binding protein 1 L homeolog Kit ELISA, insulin-like growth factor binding protein 1 Kit ELISA, IGFBP1 Kit ELISA, igfbp1a Kit ELISA, igfbp1.S Kit ELISA, igfbp1.L Kit ELISA, igfbp1 Kit ELISA, LOC100305121 Kit ELISA, Igfbp1 Kit ELISA

Sujet:

Gene Name: Insulin Like Growth Factor Binding Protein 1

Gene Aliases: IBP1, AFBP, IGF-BP25, PP12, Placental Protein 12, Amniotic Fluid Binding Protein, Alpha-Pregnancy-Associated Endometrial Globulin, Growth Hormone Independent-Binding Protein

ID gène: 282259

UniProt: P24591

Pathways: Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Growth Factor Binding