IL1RAP Kit ELISA
-
- Antigène Voir toutes IL1RAP Kits ELISA
- IL1RAP (Interleukin 1 Receptor Accessory Protein (IL1RAP))
-
Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.156 ng/mL - 10 ng/mL
- Seuil minimal de détection
- 0.156 ng/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Receptor Accessory Protein (IL1RAP). No significant cross-reactivity or interference between Interleukin 1 Receptor Accessory Protein (IL1RAP) and analogues was observed.
- Sensibilité
- 0.065 ng/mL
- Featured
- Discover our best selling IL1RAP Kit ELISA
- Top Product
- Discover our top product IL1RAP Kit ELISA
-
-
- Commentaires
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Accessory Protein (IL1RAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Accessory Protein (IL1RAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Accessory Protein (IL1RAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Accessory Protein (IL1RAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Receptor Accessory Protein (IL1RAP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Receptor Accessory Protein (IL1RAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
-
- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
-
- Antigène Voir toutes IL1RAP Kits ELISA
- IL1RAP (Interleukin 1 Receptor Accessory Protein (IL1RAP))
- Autre désignation
- Interleukin 1 Receptor Accessory Protein (IL1RAP Produits)
- Synonymes
- IL1RAP Kit ELISA, il-1racp Kit ELISA, il-racp Kit ELISA, il1r3 Kit ELISA, C3orf13 Kit ELISA, IL-1RAcP Kit ELISA, IL1R3 Kit ELISA, 6430709H04Rik Kit ELISA, AI255955 Kit ELISA, AV239853 Kit ELISA, IL-1RAcp Kit ELISA, Il1racpb Kit ELISA, interleukin 1 receptor accessory protein Kit ELISA, IL1RAP Kit ELISA, il1rap Kit ELISA, il-1racp Kit ELISA, Il1rap Kit ELISA
- Sujet
-
Gene Name: Interleukin 1 Receptor Accessory Protein
Gene Aliases: IL-1RAcP, C3orf13, IL1R3, Interleukin-1 receptor 3
- UniProt
- Q9NPH3
- Pathways
- Signalisation NF-kappaB, Growth Factor Binding
-