IL-34 Kit CLIA

N° du produit ABIN5655962

Détail du produit IL-34 Kit CLIA

Antigène: IL-34 (IL34) (Interleukin 34 (IL34))

Reactivité: Souris

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 3.91 pg/mL - 1000 pg/mL

Seuil minimal de détection: 3.91 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Interleukin 34 (IL34). No significant cross-reactivity or interference between Interleukin 34 (IL34) and analogues was observed.

Sensibilité: 0.53 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 34 (IL34). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 34 (IL34). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 34 (IL34) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 34 (IL34) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 34 (IL34) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: IL-34 (IL34) (Interleukin 34 (IL34))

Autre désignation: Interleukin 34 (IL34 Produits)

Synonymes: C16orf77 Kit CLIA, IL-34 Kit CLIA, 2010004A03Rik Kit CLIA, AI593503 Kit CLIA, C18H16ORF77 Kit CLIA, interleukin 34 Kit CLIA, IL34 Kit CLIA, Il34 Kit CLIA

Sujet:

Gene Name: Interleukin 34

Gene Aliases: C16orf77

ID gène: 76527

UniProt: Q8R1R4

Pathways: Signalisation RTK