Cytokeratin 18 Kit ELISA

N° du produit ABIN5656187

Détail du produit Cytokeratin 18 Kit ELISA

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.12 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.12 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Keratin 18 (KRT18). No significant cross-reactivity or interference between Keratin 18 (KRT18) and analogues was observed.

Sensibilité: 32 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 18 (KRT18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 18 (KRT18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 18 (KRT18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Keratin 18 (KRT18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Keratin 18 (KRT18) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Keratin 18 (KRT18) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Autre désignation: Keratin 18 (KRT18 Produits)

Synonymes: CK18 Kit ELISA, K18 Kit ELISA, Krt1-18 Kit ELISA, CYK18 Kit ELISA, DreK18 Kit ELISA, cb83 Kit ELISA, dapk1 Kit ELISA, sb:cb83 Kit ELISA, wu:fa13f02 Kit ELISA, wu:fb36b04 Kit ELISA, krt18 Kit ELISA, MGC64569 Kit ELISA, MGC75922 Kit ELISA, KRT18 Kit ELISA, CK-18-A Kit ELISA, K18-A Kit ELISA, NC-11 Kit ELISA, cyk18 Kit ELISA, k18 Kit ELISA, krt18-a Kit ELISA, krt18-b Kit ELISA, krt18a Kit ELISA, xkendob Kit ELISA, keratin 18 Kit ELISA, keratin 18, type I L homeolog Kit ELISA, keratin 18, type I Kit ELISA, keratin, type I cytoskeletal 18 Kit ELISA, K18, simple type I keratin Kit ELISA, Keratin, type I cytoskeletal 18 Kit ELISA, keratin 18, type I S homeolog Kit ELISA, Krt18 Kit ELISA, KRT18 Kit ELISA, krt18 Kit ELISA, krt18.L Kit ELISA, LOC700569 Kit ELISA, LOC100136778 Kit ELISA, k1c18 Kit ELISA, LOC100008885 Kit ELISA, krt18.S Kit ELISA

Sujet:

Gene Name: Keratin 18

Gene Aliases: CK18, CYK18, K18, Cytokeratin 18, Cell proliferation-inducing gene 46 protein, Keratin, type I cytoskeletal 18

ID gène: 16668

UniProt: P05784

Pathways: Apoptose, Caspase Cascade in Apoptosis