LIPE Kit ELISA

N° du produit ABIN5656437

Détail du produit LIPE Kit ELISA

Antigène: LIPE (Lipase, Hormone-Sensitive (LIPE))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Lipase, Hormone Sensitive (LIPE). No significant cross-reactivity or interference between Lipase, Hormone Sensitive (LIPE) and analogues was observed.

Sensibilité: 0.065 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Hormone Sensitive (LIPE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase, Hormone Sensitive (LIPE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase, Hormone Sensitive (LIPE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipase, Hormone Sensitive (LIPE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipase, Hormone Sensitive (LIPE) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipase, Hormone Sensitive (LIPE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: LIPE (Lipase, Hormone-Sensitive (LIPE))

Autre désignation: Lipase, Hormone Sensitive (LIPE Produits)

Synonymes: ADAP Kit ELISA, PRO0823 Kit ELISA, SLAP-130 Kit ELISA, HSL Kit ELISA, LHS Kit ELISA, hsl Kit ELISA, LIPE Kit ELISA, 4933403G17Rik Kit ELISA, A Kit ELISA, CG11055 Kit ELISA, Dmel\CG11055 Kit ELISA, anon-56Ea Kit ELISA, dHSL Kit ELISA, lipe Kit ELISA, si:dkey-3n22.7 Kit ELISA, FYN binding protein 1 Kit ELISA, lipase E, hormone sensitive type Kit ELISA, lipase, hormone-sensitive Kit ELISA, lipase, hormone-sensitive L homeolog Kit ELISA, lipase, hormone sensitive Kit ELISA, Hormone-sensitive lipase Kit ELISA, lipase, hormone-sensitive a Kit ELISA, FYB1 Kit ELISA, LIPE Kit ELISA, Lipe Kit ELISA, lipe Kit ELISA, lipe.L Kit ELISA, Hsl Kit ELISA, lipea Kit ELISA

Sujet:

Gene Name: Lipase, Hormone Sensitive

Gene Aliases: HSL, LHS, Hormone Sensitive Lipase

Pathways: AMPK Signaling, Monocarboxylic Acid Catabolic Process, Lipid Metabolism