LDLRAP1 Kit ELISA

N° du produit ABIN5656489

Détail du produit LDLRAP1 Kit ELISA

Antigène: LDLRAP1 (Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1). No significant cross-reactivity or interference between Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) and analogues was observed.

Sensibilité: 0.067 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: LDLRAP1 (Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1))

Autre désignation: Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1 Produits)

Synonymes: ARH Kit ELISA, ARH1 Kit ELISA, ARH2 Kit ELISA, FHCB1 Kit ELISA, FHCB2 Kit ELISA, AA691260 Kit ELISA, Arh Kit ELISA, Arh1 Kit ELISA, RGD1563417 Kit ELISA, arh Kit ELISA, arh1 Kit ELISA, arh2 Kit ELISA, fhcb1 Kit ELISA, fhcb2 Kit ELISA, xptb Kit ELISA, LDLRAP1 Kit ELISA, ldlrap1 Kit ELISA, sb:cb50 Kit ELISA, zgc:56121 Kit ELISA, zgc:158745 Kit ELISA, low density lipoprotein receptor adaptor protein 1 Kit ELISA, low density lipoprotein receptor adaptor protein 1 L homeolog Kit ELISA, low density lipoprotein receptor adaptor protein 1 S homeolog Kit ELISA, low density lipoprotein receptor adaptor protein 1a Kit ELISA, low density lipoprotein receptor adaptor protein 1b Kit ELISA, LDLRAP1 Kit ELISA, Ldlrap1 Kit ELISA, ldlrap1 Kit ELISA, ldlrap1.L Kit ELISA, ldlrap1.S Kit ELISA, ldlrap1a Kit ELISA, ldlrap1b Kit ELISA

Sujet:

Gene Name: Low Density Lipoprotein Receptor Adaptor Protein 1

Gene Aliases: ARH1, ARH, ARH2, FHCB1, FHCB2, Autosomal recessive hypercholesterolemia protein

ID gène: 100017

UniProt: Q8C142

Pathways: Lipid Metabolism