MIF Kit ELISA

N° du produit ABIN5656603

Détail du produit MIF Kit ELISA

Antigène: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Macrophage Migration Inhibitory Factor (MIF). No significant cross-reactivity or interference between Macrophage Migration Inhibitory Factor (MIF) and analogues was observed.

Sensibilité: 12.3 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Migration Inhibitory Factor (MIF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Migration Inhibitory Factor (MIF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Migration Inhibitory Factor (MIF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Migration Inhibitory Factor (MIF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Migration Inhibitory Factor (MIF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Migration Inhibitory Factor (MIF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Autre désignation: Macrophage Migration Inhibitory Factor (MIF Produits)

Synonymes: mif Kit ELISA, Mif Kit ELISA, gif Kit ELISA, glif Kit ELISA, mmif Kit ELISA, LOC100136498 Kit ELISA, LOC100284350 Kit ELISA, LOC100284546 Kit ELISA, GIF Kit ELISA, Glif Kit ELISA, GLIF Kit ELISA, MMIF Kit ELISA, macrophage migration inhibitory factor L homeolog Kit ELISA, macrophage migration inhibitory factor Kit ELISA, macrophage migration inhibitory factor (glycosylation-inhibiting factor) Kit ELISA, mif.L Kit ELISA, mif Kit ELISA, MIF Kit ELISA, Mif Kit ELISA, PHATRDRAFT_49660 Kit ELISA, LOC100136498 Kit ELISA, cl405_1 Kit ELISA, LOC100284546 Kit ELISA

Sujet:

Gene Name: Macrophage Migration Inhibitory Factor

Gene Aliases: GIF, GLIF, MMIF, Glycosylation-Inhibiting Factor, L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase

ID gène: 17319

UniProt: P34884

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling