HLAG Kit ELISA

N° du produit ABIN5656620

Détail du produit HLAG Kit ELISA

Antigène: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.38 ng/mL - 24 ng/mL

Seuil minimal de détection: 0.38 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Major Histocompatibility Complex Class I G (MHCG). No significant cross-reactivity or interference between Major Histocompatibility Complex Class I G (MHCG) and analogues was observed.

Sensibilité: 0.17 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class I G (MHCG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class I G (MHCG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class I G (MHCG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Major Histocompatibility Complex Class I G (MHCG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Major Histocompatibility Complex Class I G (MHCG) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Major Histocompatibility Complex Class I G (MHCG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Autre désignation: Major Histocompatibility Complex Class I G (HLAG Produits)

Synonymes: MHC-G Kit ELISA, B-F Kit ELISA, B-F-S04 Kit ELISA, B-F-S05 Kit ELISA, B-F-S06 Kit ELISA, B-F-S07 Kit ELISA, B-FI Kit ELISA, B-FIV Kit ELISA, BF2 Kit ELISA, BFa2 Kit ELISA, BFw-03 Kit ELISA, BFw-05 Kit ELISA, BFz-01 Kit ELISA, major histocompatibility complex, class I, G Kit ELISA, MHC BF1 class I Kit ELISA, HLA-G Kit ELISA, BF1 Kit ELISA

Sujet:

Gene Name: Major Histocompatibility Complex Class I G

Gene Aliases: HLA-G, MHC-G, HLAG, Leukocyte Antigen G, b2 microglobulin, HLA class I histocompatibility antigen, alpha chain G

ID gène: 3135

UniProt: P17693

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints