METAP2 Kit ELISA

N° du produit ABIN5656865

Détail du produit METAP2 Kit ELISA

Antigène: METAP2 (Methionyl Aminopeptidase 2 (METAP2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Methionyl Aminopeptidase 2 (METAP2). No significant cross-reactivity or interference between Methionyl Aminopeptidase 2 (METAP2) and analogues was observed.

Sensibilité: 0.058 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methionyl Aminopeptidase 2 (METAP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methionyl Aminopeptidase 2 (METAP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methionyl Aminopeptidase 2 (METAP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methionyl Aminopeptidase 2 (METAP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Methionyl Aminopeptidase 2 (METAP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Methionyl Aminopeptidase 2 (METAP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: METAP2 (Methionyl Aminopeptidase 2 (METAP2))

Autre désignation: Methionyl Aminopeptidase 2 (METAP2 Produits)

Synonymes: metap2 Kit ELISA, MGC53792 Kit ELISA, METAP2 Kit ELISA, GB13458 Kit ELISA, DDBDRAFT_0190923 Kit ELISA, DDBDRAFT_0304991 Kit ELISA, DDB_0190923 Kit ELISA, DDB_0304991 Kit ELISA, 4930584B20Rik Kit ELISA, A930035J23Rik Kit ELISA, AI047573 Kit ELISA, AL024412 Kit ELISA, AU014659 Kit ELISA, Amp2 Kit ELISA, Mnpep Kit ELISA, p67 Kit ELISA, p67eIF2 Kit ELISA, MAP2 Kit ELISA, MNPEP Kit ELISA, wu:fb98h06 Kit ELISA, zgc:66250 Kit ELISA, methionyl aminopeptidase 2 L homeolog Kit ELISA, methionyl aminopeptidase 2 Kit ELISA, methionine aminopeptidase 2 Kit ELISA, methionine aminopeptidase Kit ELISA, methionyl aminopeptidase 2 S homeolog Kit ELISA, methionyl aminopeptidase 2b Kit ELISA, metap2.L Kit ELISA, METAP2 Kit ELISA, metap2 Kit ELISA, LOC551771 Kit ELISA, CAALFM_C604080WA Kit ELISA, Metap2 Kit ELISA, metap2.S Kit ELISA, metap2b Kit ELISA

Sujet:

Gene Name: Methionyl Aminopeptidase 2

Gene Aliases: MNPEP, p67, p67eIF2, Peptidase M, Initiation factor 2-associated 67 kDa glycoprotein

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling