Midkine Kit ELISA

N° du produit ABIN5656905

Détail du produit Midkine Kit ELISA

Antigène: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Midkine (MK). No significant cross-reactivity or interference between Midkine (MK) and analogues was observed.

Sensibilité: 0.054 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Midkine (MK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Midkine (MK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Midkine (MK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Midkine (MK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Midkine (MK) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Midkine (MK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Autre désignation: Midkine (MDK Produits)

Synonymes: ARAP Kit ELISA, MK Kit ELISA, NEGF2 Kit ELISA, Mek Kit ELISA, MK-B Kit ELISA, PTF-alpha-1 Kit ELISA, mdk Kit ELISA, midkine Kit ELISA, negf2 Kit ELISA, RIHB Kit ELISA, cb518 Kit ELISA, fb79b05 Kit ELISA, fj33b02 Kit ELISA, fj48a12 Kit ELISA, mdk2 Kit ELISA, plei2 Kit ELISA, wu:fb79b05 Kit ELISA, wu:fj33b02 Kit ELISA, wu:fj48a12 Kit ELISA, MK-A Kit ELISA, PTF-alpha-2 Kit ELISA, X-PTF-alpha1 Kit ELISA, XMK Kit ELISA, mdk-a Kit ELISA, mdk-b Kit ELISA, midkine Kit ELISA, midkine L homeolog Kit ELISA, midkine (neurite growth-promoting factor 2) Kit ELISA, midkine b Kit ELISA, midkine S homeolog Kit ELISA, MDK Kit ELISA, Mdk Kit ELISA, mdk.L Kit ELISA, mdkb Kit ELISA, mdk.S Kit ELISA

Sujet:

Gene Name: Midkine

Gene Aliases: MDK, ARAP, NEGF2, Neurite Growth-Promoting Factor 2, Amphiregulin-associated protein, Midgestation and kidney protein, Neurite outgrowth-promoting factor 2

ID gène: 17242

UniProt: P12025

Pathways: Signalisation RTK, M Phase, Skeletal Muscle Fiber Development