MFGE8 Kit ELISA

N° du produit ABIN5656910

Détail du produit MFGE8 Kit ELISA

Antigène: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.62 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Milk Fat Globule EGF Factor 8 (MFGE8). No significant cross-reactivity or interference between Milk Fat Globule EGF Factor 8 (MFGE8) and analogues was observed.

Sensibilité: 5.9 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Milk Fat Globule EGF Factor 8 (MFGE8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Milk Fat Globule EGF Factor 8 (MFGE8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Milk Fat Globule EGF Factor 8 (MFGE8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Milk Fat Globule EGF Factor 8 (MFGE8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Milk Fat Globule EGF Factor 8 (MFGE8) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Milk Fat Globule EGF Factor 8 (MFGE8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Autre désignation: Milk Fat Globule EGF Factor 8 (MFGE8 Produits)

Synonymes: BA46 Kit ELISA, EDIL1 Kit ELISA, HMFG Kit ELISA, HsT19888 Kit ELISA, MFG-E8 Kit ELISA, MFGM Kit ELISA, OAcGD3S Kit ELISA, SED1 Kit ELISA, SPAG10 Kit ELISA, hP47 Kit ELISA, ba46 Kit ELISA, edil1 Kit ELISA, oacgd3s Kit ELISA, MGC75581 Kit ELISA, hst19888 Kit ELISA, MGC145109 Kit ELISA, MFGE8 Kit ELISA, mfge8 Kit ELISA, zgc:154157 Kit ELISA, MGC99228 Kit ELISA, AA408458 Kit ELISA, AI325141 Kit ELISA, Mfgm Kit ELISA, P47 Kit ELISA, lactadherin Kit ELISA, AGS Kit ELISA, MFGME8 Kit ELISA, MFGMP-E8 Kit ELISA, im:6895406 Kit ELISA, mfge8l Kit ELISA, zgc:110846 Kit ELISA, milk fat globule-EGF factor 8 protein Kit ELISA, milk fat globule-EGF factor 8 protein b Kit ELISA, milk fat globule-EGF factor 8 protein L homeolog Kit ELISA, milk fat globule-EGF factor 8 protein a Kit ELISA, MFGE8 Kit ELISA, mfge8 Kit ELISA, mfge8b Kit ELISA, mfge8.L Kit ELISA, Mfge8 Kit ELISA, mfge8a Kit ELISA

Sujet:

Gene Name: Milk Fat Globule EGF Factor 8

Gene Aliases: HMFG, MFGM, SED1, BA46, EDIL1, OAcGD3S, SPAG10, hP47, Medin, Lactadherin, Sperm Associated Antigen 10, Sperm Surface Protein hP47, Milk fat globule-EGF factor 8

ID gène: 25277

UniProt: P70490

Pathways: SARS-CoV-2 Protein Interactome