Moesin Kit ELISA

N° du produit ABIN5656931

Détail du produit Moesin Kit ELISA

Antigène: Moesin (MSN)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Moesin (MSN). No significant cross-reactivity or interference between Moesin (MSN) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Moesin (MSN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Moesin (MSN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Moesin (MSN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Moesin (MSN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Moesin (MSN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Moesin (MSN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Moesin (MSN)

Autre désignation: Moesin (MSN Produits)

Synonymes: CG10701 Kit ELISA, D17 Kit ELISA, Dmel\\CG10701 Kit ELISA, Dmoe Kit ELISA, EMR1 Kit ELISA, ERM Kit ELISA, Emr1 Kit ELISA, anon-WO03040301.155 Kit ELISA, anon-WO03040301.157 Kit ELISA, anon-WO03040301.159 Kit ELISA, anon-WO03040301.161 Kit ELISA, dMoe Kit ELISA, gma Kit ELISA, l(1)G0067 Kit ELISA, l(1)G0323 Kit ELISA, l(1)G0404 Kit ELISA, l(1)G0415 Kit ELISA, moe Kit ELISA, moesin/ezrin/radixin homolog mRNA Kit ELISA, cb567 Kit ELISA, rdx Kit ELISA, wu:fb14b03 Kit ELISA, zgc:85640 Kit ELISA, MSN Kit ELISA, C78546 Kit ELISA, moesin Kit ELISA, Moesin Kit ELISA, moesin a Kit ELISA, moesin L homeolog Kit ELISA, MSN Kit ELISA, Moe Kit ELISA, msna Kit ELISA, msn.L Kit ELISA, moes Kit ELISA, Msn Kit ELISA

Sujet:

Gene Name: Moesin

Gene Aliases: Membrane-Organizing Extension Spike Protein

ID gène: 4478

UniProt: P26038

Pathways: Asymmetric Protein Localization