SMAD1 Kit ELISA

N° du produit ABIN5656963

Détail du produit SMAD1 Kit ELISA

Antigène: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Mothers Against Decapentaplegic Homolog 1 (Smad1). No significant cross-reactivity or interference between Mothers Against Decapentaplegic Homolog 1 (Smad1) and analogues was observed.

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mothers Against Decapentaplegic Homolog 1 (Smad1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mothers Against Decapentaplegic Homolog 1 (Smad1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mothers Against Decapentaplegic Homolog 1 (Smad1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mothers Against Decapentaplegic Homolog 1 (Smad1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 1 (Smad1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 1 (Smad1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Autre désignation: Mothers Against Decapentaplegic Homolog 1 (SMAD1 Produits)

Synonymes: MLP Kit ELISA, Xmad1 Kit ELISA, Xsmad1 Kit ELISA, bsp1 Kit ELISA, jv4-1 Kit ELISA, jv41 Kit ELISA, madh1 Kit ELISA, madr1 Kit ELISA, smad-1 Kit ELISA, MADH1 Kit ELISA, fb39h09 Kit ELISA, wu:fb39h09 Kit ELISA, wu:fb97e04 Kit ELISA, 2/23 Kit ELISA, CG12399 Kit ELISA, Dmel\\CG12399 Kit ELISA, E(zen)2 Kit ELISA, En(vvl) Kit ELISA, MAD Kit ELISA, Mat Kit ELISA, P-Mad Kit ELISA, P-mad Kit ELISA, PMad Kit ELISA, Smad Kit ELISA, Smad1 Kit ELISA, apg Kit ELISA, c28 Kit ELISA, dMAD Kit ELISA, dMad Kit ELISA, l(2)K00237 Kit ELISA, l(2)k00237 Kit ELISA, mad Kit ELISA, p-Mad Kit ELISA, pMAD Kit ELISA, pMad Kit ELISA, pSmad Kit ELISA, BSP-1 Kit ELISA, BSP1 Kit ELISA, JV4-1 Kit ELISA, JV41 Kit ELISA, MADR1 Kit ELISA, AI528653 Kit ELISA, Madh1 Kit ELISA, Madr1 Kit ELISA, Mlp1 Kit ELISA, MusMLP Kit ELISA, smad1 Kit ELISA, xmad Kit ELISA, SMAD family member 1 Kit ELISA, Mothers against dpp Kit ELISA, SMAD family member 1 L homeolog Kit ELISA, smad1 Kit ELISA, SMAD1 Kit ELISA, Mad Kit ELISA, Smad1 Kit ELISA, smad1.L Kit ELISA

ID gène: 17125

UniProt: P70340

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development