MUSK Kit ELISA

N° du produit ABIN5657020

Détail du produit MUSK Kit ELISA

Antigène: MUSK (Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK). No significant cross-reactivity or interference between Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) and analogues was observed.

Sensibilité: 0.061 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MUSK (Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK))

Autre désignation: Muscle, Skeletal, Receptor Tyrosine Kinase (MUSK Produits)

Synonymes: unp Kit ELISA, fj98e12 Kit ELISA, zgc:63467 Kit ELISA, wu:fj98e12 Kit ELISA, si:dz46m7.2 Kit ELISA, si:dz57f23.3 Kit ELISA, si:busm1-57f23.3 Kit ELISA, musk-A Kit ELISA, MuSK Kit ELISA, MUSK Kit ELISA, Mdk4 Kit ELISA, Mlk Kit ELISA, Nsk1 Kit ELISA, Nsk2 Kit ELISA, Nsk3 Kit ELISA, muscle Kit ELISA, muscle associated receptor tyrosine kinase Kit ELISA, muscle, skeletal, receptor tyrosine kinase Kit ELISA, muscle associated receptor tyrosine kinase S homeolog Kit ELISA, MUSK Kit ELISA, musk Kit ELISA, musk.S Kit ELISA, Musk Kit ELISA

Sujet:

Gene Name: Muscle, Skeletal, Receptor Tyrosine Kinase

Gene Aliases: Muscle-specific tyrosine-protein kinase receptor, Muscle-specific kinase receptor

ID gène: 18198

UniProt: Q61006

Pathways: Signalisation RTK, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development