NLRC4 Kit ELISA

N° du produit ABIN5657418

Détail du produit NLRC4 Kit ELISA

Antigène: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of NLR Family, CARD Domain Containing Protein 4 (NLRC4). No significant cross-reactivity or interference between NLR Family, CARD Domain Containing Protein 4 (NLRC4) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NLR Family, CARD Domain Containing Protein 4 (NLRC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to NLR Family, CARD Domain Containing Protein 4 (NLRC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NLR Family, CARD Domain Containing Protein 4 (NLRC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NLR Family, CARD Domain Containing Protein 4 (NLRC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NLR Family, CARD Domain Containing Protein 4 (NLRC4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NLR Family, CARD Domain Containing Protein 4 (NLRC4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))

Autre désignation: NLR Family, CARD Domain Containing Protein 4 (NLRC4 Produits)

Synonymes: CARD12 Kit ELISA, CLAN Kit ELISA, CLAN1 Kit ELISA, CLANA Kit ELISA, CLANB Kit ELISA, CLANC Kit ELISA, CLAND Kit ELISA, CLR2.1 Kit ELISA, IPAF Kit ELISA, 9530011P19Rik Kit ELISA, Card12 Kit ELISA, NLR family CARD domain containing 4 Kit ELISA, NLR family, CARD domain containing 4 Kit ELISA, NLRC4 Kit ELISA, Nlrc4 Kit ELISA

Sujet:

Gene Name: NLR Family, CARD Domain Containing Protein 4

Gene Aliases: CARD12, CLAN1, CLANA, CLANB, CLANC, CLAND, CLR2.1, IPAF, Ice protease-activating factor, Caspase Recruitment Domain Family Member 12, NOD-Like Receptor C4

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome