NUP62 Kit ELISA

N° du produit ABIN5657474

Détail du produit NUP62 Kit ELISA

Antigène: NUP62 (Nucleoporin 62kDa (NUP62))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Nucleoporin 62 kDa (NUP62). No significant cross-reactivity or interference between Nucleoporin 62 kDa (NUP62) and analogues was observed.

Sensibilité: 0.112 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nucleoporin 62 kDa (NUP62). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nucleoporin 62 kDa (NUP62). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nucleoporin 62 kDa (NUP62), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nucleoporin 62 kDa (NUP62) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nucleoporin 62kDa (NUP62) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nucleoporin 62kDa (NUP62) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: NUP62 (Nucleoporin 62kDa (NUP62))

Autre désignation: Nucleoporin 62kDa (NUP62 Produits)

Synonymes: IBSN Kit ELISA, SNDI Kit ELISA, p62 Kit ELISA, NUP62 Kit ELISA, AA589433 Kit ELISA, AI426861 Kit ELISA, AI790512 Kit ELISA, AU045898 Kit ELISA, D7Ertd649e Kit ELISA, Nupc1 Kit ELISA, CG6251 Kit ELISA, Dmel\\CG6251 Kit ELISA, DDBDRAFT_0167902 Kit ELISA, DDBDRAFT_0237712 Kit ELISA, DDB_0167902 Kit ELISA, DDB_0237712 Kit ELISA, nup62 Kit ELISA, Np62 Kit ELISA, nucleoporin 62 Kit ELISA, hypothetical protein Kit ELISA, nucleoporin 62kDa Kit ELISA, Nucleoporin 62kD Kit ELISA, predicted protein Kit ELISA, nuclear pore protein Kit ELISA, Nup62 nuclear pore complex glycoprotein p62-related protein (IC) Kit ELISA, Nuclear pore glycoprotein p62 Kit ELISA, NUP62 Kit ELISA, PGTG_03077 Kit ELISA, Nup62 Kit ELISA, nup62 Kit ELISA

Sujet:

Gene Name: Nucleoporin 62 kDa

Gene Aliases: IBSN, p62, SNDI, Nuclear Pore Glycoprotein p62

ID gène: 23636

UniProt: P37198

Pathways: EGFR Signaling Pathway, SARS-CoV-2 Protein Interactome