NOD2 Kit CLIA

N° du produit ABIN5657479

Détail du produit NOD2 Kit CLIA

Antigène: NOD2 (Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 468.75 pg/mL - 30000 pg/mL

Seuil minimal de détection: 468.75 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2). No significant cross-reactivity or interference between Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) and analogues was observed.

Sensibilité: 17.4 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: NOD2 (Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2))

Autre désignation: Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2 Produits)

Synonymes: ACUG Kit CLIA, BLAU Kit CLIA, CARD15 Kit CLIA, CD Kit CLIA, CLR16.3 Kit CLIA, IBD1 Kit CLIA, NLRC2 Kit CLIA, NOD2B Kit CLIA, PSORAS1 Kit CLIA, Card15 Kit CLIA, F830032C23Rik Kit CLIA, Nlrc2 Kit CLIA, caspase recruitment domain 15 protein Kit CLIA, nucleotide binding oligomerization domain containing 2 Kit CLIA, nucleotide-binding oligomerization domain containing 2 Kit CLIA, TTHERM_01951320 Kit CLIA, NOD2 Kit CLIA, Nod2 Kit CLIA

Sujet:

Gene Name: Nucleotide Binding Oligomerization Domain Containing Protein 2

Gene Aliases: CD, ACUG, BLAU, CARD15, CLR16.3, IBD1, NLRC2, NOD2B, PSORAS1, Caspase recruitment domain-containing protein 15, Inflammatory bowel disease protein 1

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome