MGMT Kit ELISA

N° du produit ABIN5657482

Le kit ELISA MGMT (ABIN5657482) Rat est un kit ELISA Colorimetric conçu pour quantifier Rat MGMT.

Aperçu rapide pour MGMT Kit ELISA (ABIN5657482)

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.12 pg/mL - 5000 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Détail du produit MGMT Kit ELISA

Seuil minimal de détection: 78.12 pg/mL

Fonction: Rat O-6-Methylguanine DNA Methyltransferase (MGMT) ELISA Kit for detecting MGMT in tissue homogenates

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of O-6-Methylguanine DNA Methyltransferase (MGMT). No significant cross-reactivity or interference between O-6-Methylguanine DNA Methyltransferase (MGMT) and analogues was observed.

Sensibilité: 27 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Matériel non inclus:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to O-6-Methylguanine DNA Methyltransferase (MGMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to O-6-Methylguanine DNA Methyltransferase (MGMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain O-6-Methylguanine DNA Methyltransferase (MGMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of O-6-Methylguanine DNA Methyltransferase (MGMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level O-6-Methylguanine DNA Methyltransferase (MGMT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level O-6-Methylguanine DNA Methyltransferase (MGMT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Autre désignation: O-6-Methylguanine DNA Methyltransferase

Sujet: Gene Aliases: Methylated-DNA--Protein-Cysteine Methyltransferase, 6-O-methylguanine-DNA methyltransferase, Methylated-DNA--protein-cysteine methyltransferase

ID gène: 25332

UniProt: P24528

Pathways: Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus