PCNT Kit ELISA

N° du produit ABIN5657696

Détail du produit PCNT Kit ELISA

Antigène: PCNT (Pericentrin (PCNT))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Pericentrin (PCNT). No significant cross-reactivity or interference between Pericentrin (PCNT) and analogues was observed.

Sensibilité: 0.119 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pericentrin (PCNT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pericentrin (PCNT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pericentrin (PCNT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pericentrin (PCNT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pericentrin (PCNT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pericentrin (PCNT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PCNT (Pericentrin (PCNT))

Autre désignation: Pericentrin (PCNT Produits)

Synonymes: AKAP450 Kit ELISA, CG13459 Kit ELISA, CG18648 Kit ELISA, CG33957 Kit ELISA, CG6735 Kit ELISA, CP309 Kit ELISA, Cp309 Kit ELISA, D-PLP Kit ELISA, DPLP Kit ELISA, Dmel\\CG33957 Kit ELISA, Dplp Kit ELISA, PACT Kit ELISA, PLP Kit ELISA, Plp Kit ELISA, d-plp Kit ELISA, dPLP Kit ELISA, dplp Kit ELISA, l(3)s2172 Kit ELISA, pPlp Kit ELISA, KEN Kit ELISA, MOPD2 Kit ELISA, PCN Kit ELISA, PCNT2 Kit ELISA, PCNTB Kit ELISA, PCTN2 Kit ELISA, SCKL4 Kit ELISA, DIP2A Kit ELISA, AW476095 Kit ELISA, C86676 Kit ELISA, Pcnt2 Kit ELISA, kendrin Kit ELISA, m239Asp Kit ELISA, m275Asp Kit ELISA, pericentrin Kit ELISA, Pericentrin-like protein Kit ELISA, pericentrin (kendrin) Kit ELISA, LOC100055203 Kit ELISA, Plp Kit ELISA, PCNT Kit ELISA, pcnt Kit ELISA, Pcnt Kit ELISA

Sujet:

Gene Name: Pericentrin

Gene Aliases: KEN, PCN, PCNT2, PCNTB, PCTN2, SCKL4, Kendrin, Seckel Syndrome 4, Pericentrin-B

ID gène: 5116

UniProt: O95613

Pathways: Sensory Perception of Sound, M Phase, SARS-CoV-2 Protein Interactome