PPARA Kit ELISA

N° du produit ABIN5657732

Détail du produit PPARA Kit ELISA

Antigène: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Peroxisome Proliferator Activated Receptor Alpha (PPARa). No significant cross-reactivity or interference between Peroxisome Proliferator Activated Receptor Alpha (PPARa) and analogues was observed.

Sensibilité: 0.112 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Alpha (PPARa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisome Proliferator Activated Receptor Alpha (PPARa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisome Proliferator Activated Receptor Alpha (PPARa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxisome Proliferator Activated Receptor Alpha (PPARa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Alpha (PPARa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Alpha (PPARa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))

Autre désignation: Peroxisome Proliferator Activated Receptor Alpha (PPARA Produits)

Synonymes: NR1C1 Kit ELISA, PPAR Kit ELISA, PPARalpha Kit ELISA, hPPAR Kit ELISA, LOC100136415 Kit ELISA, PPARALPHA Kit ELISA, nr1c1 Kit ELISA, xPPARalpha Kit ELISA, xppara Kit ELISA, 4933429D07Rik Kit ELISA, AW742785 Kit ELISA, Nr1c1 Kit ELISA, PPAR-alpha Kit ELISA, Ppar Kit ELISA, peroxisome proliferator activated receptor alpha Kit ELISA, peroxisome proliferator-activated receptor alpha Kit ELISA, peroxisome proliferator activated receptor alpha L homeolog Kit ELISA, PPARA Kit ELISA, LOC100136415 Kit ELISA, ppara.L Kit ELISA, Ppara Kit ELISA, ppara Kit ELISA

Sujet:

Gene Name: Peroxisome Proliferator Activated Receptor Alpha

Gene Aliases: NR1C1, PPAR-A, HPPAR, Nuclear Receptor Subfamily 1 Group C Member 1

ID gène: 5465

UniProt: Q07869

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C