PDE4D Kit ELISA

N° du produit ABIN5657768

Détail du produit PDE4D Kit ELISA

Antigène: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Phosphodiesterase 4D, cAMP Specific (PDE4D). No significant cross-reactivity or interference between Phosphodiesterase 4D, cAMP Specific (PDE4D) and analogues was observed.

Sensibilité: 0.112 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphodiesterase 4D, cAMP Specific (PDE4D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphodiesterase 4D, cAMP Specific (PDE4D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphodiesterase 4D, cAMP Specific (PDE4D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphodiesterase 4D, cAMP Specific (PDE4D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphodiesterase 4D, cAMP Specific (PDE4D) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphodiesterase 4D, cAMP Specific (PDE4D) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))

Autre désignation: Phosphodiesterase 4D, cAMP Specific (PDE4D Produits)

Synonymes: ACRDYS2 Kit ELISA, DPDE3 Kit ELISA, HSPDE4D Kit ELISA, PDE43 Kit ELISA, PDE4DN2 Kit ELISA, STRK1 Kit ELISA, CMYA2 Kit ELISA, MMGL Kit ELISA, 9630011N22Rik Kit ELISA, Dpde3 Kit ELISA, 4732458A06Rik Kit ELISA, 9430063L05Rik Kit ELISA, C87016 Kit ELISA, D130016K21Rik Kit ELISA, D3Bwg1078e Kit ELISA, Usmg4 Kit ELISA, mKIAA0454 Kit ELISA, si:dkey-190g11.2 Kit ELISA, phosphodiesterase 4D Kit ELISA, phosphodiesterase 4D interacting protein Kit ELISA, phosphodiesterase 4D, cAMP specific Kit ELISA, phosphodiesterase 4D, cAMP-specific Kit ELISA, phosphodiesterase 4D interacting protein (myomegalin) Kit ELISA, PDE4D Kit ELISA, PDE4DIP Kit ELISA, pde4d Kit ELISA, Pde4dip Kit ELISA, Pde4d Kit ELISA

Sujet:

Gene Name: Phosphodiesterase 4D, cAMP Specific

Gene Aliases: DPDE3, PDE43, Dunce(Drosophila)Homolog Phosphodiesterase E3, cAMP-specific 3',5'-cyclic phosphodiesterase 4D

ID gène: 5144

UniProt: Q08499

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling