Phospholipase D Kit ELISA

N° du produit ABIN5657812

Détail du produit Phospholipase D Kit ELISA

Antigène: Phospholipase D (PLD)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.12 ng/mL - 200 ng/mL

Seuil minimal de détection: 3.12 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Phospholipase D (PLD). No significant cross-reactivity or interference between Phospholipase D (PLD) and analogues was observed.

Sensibilité: 1.38 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase D (PLD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase D (PLD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase D (PLD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase D (PLD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase D (PLD) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase D (PLD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Phospholipase D (PLD)

Autre désignation: Phospholipase D (PLD Produits)

Synonymes: CG12110 Kit ELISA, Dmel\\CG12110 Kit ELISA, PLD Kit ELISA, dPLD Kit ELISA, dPld Kit ELISA, pld Kit ELISA, pld1 Kit ELISA, PHOSPHOLIPASE D Kit ELISA, PHOSPHOLIPASE D P1 Kit ELISA, PHOSPHOLIPASE D ZETA 1 Kit ELISA, PHOSPHOLIPASE D ZETA1 Kit ELISA, PLD ZETA 1 Kit ELISA, PLDZ1 Kit ELISA, PLDZETA1 Kit ELISA, phospholipase D P1 Kit ELISA, Phospholipase D Kit ELISA, phospholipase D P1 Kit ELISA, phospholipase Kit ELISA, phospholipase D Kit ELISA, phospholipase d Kit ELISA, glycosylphosphatidylinositol specific phospholipase D1 Kit ELISA, Pld Kit ELISA, PLDP1 Kit ELISA, RR_RS10410 Kit ELISA, CNE01480 Kit ELISA, pld Kit ELISA, plD Kit ELISA, GPLD1 Kit ELISA

Sujet:

Gene Name: Phospholipase D

Gene Aliases: PLD1, Choline Phosphatase 1, Phosphatidylcholine-hydrolyzing phospholipase D1

ID gène: 5337

UniProt: Q13393

Pathways: Response to Water Deprivation, ER-Nucleus Signaling