PSPH Kit CLIA

N° du produit ABIN5657820

Détail du produit PSPH Kit CLIA

Antigène: PSPH (Phosphoserine Phosphatase (PSPH))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Phosphoserine Phosphatase (PSPH). No significant cross-reactivity or interference between Phosphoserine Phosphatase (PSPH) and analogues was observed.

Sensibilité: 0.061 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Phosphoserine Phosphatase (PSPH). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Phosphoserine Phosphatase (PSPH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Phosphoserine Phosphatase (PSPH) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphoserine Phosphatase (PSPH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphoserine Phosphatase (PSPH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PSPH (Phosphoserine Phosphatase (PSPH))

Autre désignation: Phosphoserine Phosphatase (PSPH Produits)

Synonymes: PSP Kit CLIA, PSPHD Kit CLIA, AI480570 Kit CLIA, PSPase Kit CLIA, PSPH Kit CLIA, psph Kit CLIA, MGC78828 Kit CLIA, zgc:112414 Kit CLIA, DDBDRAFT_0183847 Kit CLIA, DDBDRAFT_0230054 Kit CLIA, DDB_0183847 Kit CLIA, DDB_0230054 Kit CLIA, phosphoserine phosphatase Kit CLIA, phosphoserine phosphatase L homeolog Kit CLIA, PSP; O-phosphoserine phosphohydrolase; PSPase Kit CLIA, phosphoserine phosphatase SerB Kit CLIA, BPI fold containing family A member 2 Kit CLIA, PSPH Kit CLIA, Psph Kit CLIA, psph.L Kit CLIA, psph Kit CLIA, serB Kit CLIA, serB-1 Kit CLIA, LOC5568588 Kit CLIA, PSP Kit CLIA, Svir_29300 Kit CLIA, MMAH_RS03435 Kit CLIA, BPIFA2 Kit CLIA

Sujet:

Gene Name: Phosphoserine Phosphatase

Gene Aliases: PSP, L-3-phosphoserine phosphatase, O-phosphoserine phosphohydrolase

Pathways: L'effet Warburg