PCNA Kit ELISA

N° du produit ABIN5658110

Détail du produit PCNA Kit ELISA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain, Rat, Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Proliferating Cell Nuclear Antigen (PCNA). No significant cross-reactivity or interference between Proliferating Cell Nuclear Antigen (PCNA) and analogues was observed.

Sensibilité: 0.054 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proliferating Cell Nuclear Antigen (PCNA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proliferating Cell Nuclear Antigen (PCNA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proliferating Cell Nuclear Antigen (PCNA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proliferating Cell Nuclear Antigen (PCNA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proliferating Cell Nuclear Antigen (PCNA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proliferating Cell Nuclear Antigen (PCNA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: Proliferating Cell Nuclear Antigen (PCNA Produits)

Synonymes: PCNAR Kit ELISA, Pcna/cyclin Kit ELISA, pcna Kit ELISA, AO090003000846 Kit ELISA, 53/13 Kit ELISA, CG9193 Kit ELISA, DmPCNA Kit ELISA, Dmel\\CG9193 Kit ELISA, MUS209 Kit ELISA, Mus209 Kit ELISA, PCNA Kit ELISA, PCNA/mus209 Kit ELISA, Pcna Kit ELISA, dPCNA Kit ELISA, l(2)02448 Kit ELISA, l(2)56F Kit ELISA, l(2)56Fa Kit ELISA, l(2)s1534 Kit ELISA, cb16 Kit ELISA, fb36g03 Kit ELISA, wu:fa28e03 Kit ELISA, wu:fb36g03 Kit ELISA, pcna-A Kit ELISA, proliferating cell nuclear antigen Kit ELISA, DNA polymerase III sliding clamp Kit ELISA, Proliferating cell nuclear antigen Kit ELISA, Proliferating cell nuclear antigen-like Kit ELISA, proliferating cell nuclear antigen L homeolog Kit ELISA, proliferating cell nuclear antigen 1 Kit ELISA, PCNA Kit ELISA, Pcna Kit ELISA, pcna Kit ELISA, MMP_RS08810 Kit ELISA, ANI_1_512014 Kit ELISA, AOR_1_1500154 Kit ELISA, MGC53867 Kit ELISA, pcna.L Kit ELISA, pcna1 Kit ELISA, LOC106420145 Kit ELISA, pcn1 Kit ELISA

Sujet:

Gene Name: Proliferating Cell Nuclear Antigen

Gene Aliases: Cyclin

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy