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PA2G4 Kit ELISA

PA2G4 Reactivité: Humain Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5658111
  • Antigène Voir toutes PA2G4 Kits ELISA
    PA2G4 (Proliferation-Associated 2G4, 38kDa (PA2G4))
    Reactivité
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.312 ng/mL - 20 ng/mL
    Seuil minimal de détection
    0.312 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Proliferation Associated Protein 2G4 (PA2G4). No significant cross-reactivity or interference between Proliferation Associated Protein 2G4 (PA2G4) and analogues was observed.
    Sensibilité
    0.114 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proliferation Associated Protein 2G4 (PA2G4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proliferation Associated Protein 2G4 (PA2G4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proliferation Associated Protein 2G4 (PA2G4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proliferation Associated Protein 2G4 (PA2G4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proliferation Associated Protein 2G4 (PA2G4) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proliferation Associated Protein 2G4 (PA2G4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes PA2G4 Kits ELISA
    PA2G4 (Proliferation-Associated 2G4, 38kDa (PA2G4))
    Autre désignation
    Proliferation Associated Protein 2G4 (PA2G4 Produits)
    Synonymes
    EBP1 Kit ELISA, HG4-1 Kit ELISA, p38-2G4 Kit ELISA, Ebp1 Kit ELISA, pa2g4 Kit ELISA, si:dz150i12.2 Kit ELISA, wu:fb19b11 Kit ELISA, wu:ft56d05 Kit ELISA, zgc:86732 Kit ELISA, ebp1 Kit ELISA, hg4-1 Kit ELISA, p38-2g4 Kit ELISA, pa2g4-a Kit ELISA, PA2G4 Kit ELISA, DKFZp469G2132 Kit ELISA, Pa2g4 Kit ELISA, MGC81621 Kit ELISA, 38kDa Kit ELISA, AA672939 Kit ELISA, Plfap Kit ELISA, proliferation-associated 2G4 Kit ELISA, proliferation-associated 2G4, a Kit ELISA, proliferation-associated 2G4, 38kDa Kit ELISA, proliferation-associated 2G4 L homeolog Kit ELISA, proliferation-associated protein 2G4 Kit ELISA, proliferation-associated 2G4 S homeolog Kit ELISA, proliferation-associated protein 2G4 pseudogene Kit ELISA, PA2G4 Kit ELISA, Pa2g4 Kit ELISA, pa2g4a Kit ELISA, pa2g4.L Kit ELISA, pa2g4 Kit ELISA, LOC100519773 Kit ELISA, pa2g4.S Kit ELISA, LOC693641 Kit ELISA
    Sujet

    Gene Name: Proliferation Associated Protein 2G4

    Gene Aliases: EBP1, HG4-1, p38-2G4, Cell cycle protein p38-2G4 homolog, ErbB3-binding protein 1

    ID gène
    5036
    UniProt
    Q9UQ80
    Pathways
    Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Toll-Like Receptors Cascades
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