P4HB Kit ELISA

N° du produit ABIN5658197

Détail du produit P4HB Kit ELISA

Antigène: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase (PDI). No significant cross-reactivity or interference between Protein Disulfide Isomerase (PDI) and analogues was observed.

Sensibilité: 0.114 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase (PDI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase (PDI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase (PDI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Disulfide Isomerase (PDI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Disulfide Isomerase (PDI) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Disulfide Isomerase (PDI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Autre désignation: Protein Disulfide Isomerase (P4HB Produits)

Synonymes: DSI Kit ELISA, ERBA2L Kit ELISA, GIT Kit ELISA, P4Hbeta Kit ELISA, PDI Kit ELISA, PDIA1 Kit ELISA, PHDB Kit ELISA, PO4DB Kit ELISA, PO4HB Kit ELISA, PROHB Kit ELISA, PDA2 Kit ELISA, PDIP Kit ELISA, PDIR Kit ELISA, ERp59 Kit ELISA, Pdia1 Kit ELISA, Thbp Kit ELISA, p55 Kit ELISA, XPDIp Kit ELISA, pdi Kit ELISA, 1810041F13Rik Kit ELISA, AI661267 Kit ELISA, Pdip Kit ELISA, Pdipl Kit ELISA, p58 Kit ELISA, prolyl 4-hydroxylase subunit beta Kit ELISA, protein disulfide isomerase family A member 2 Kit ELISA, prolyl 4-hydroxylase, beta polypeptide Kit ELISA, protein disulfide isomerase family A member 2 L homeolog Kit ELISA, protein disulfide isomerase associated 2 Kit ELISA, prolyl 4-hydroxylase subunit beta L homeolog Kit ELISA, protein disulfide-isomerase Kit ELISA, P4HB Kit ELISA, PDIA2 Kit ELISA, P4hb Kit ELISA, pdia2.L Kit ELISA, Pdia2 Kit ELISA, p4hb.L Kit ELISA, LOC8281065 Kit ELISA

Sujet:

Gene Name: Protein Disulfide Isomerase

Gene Aliases: P4HB, ERBA2L, PDIA1, PO4DB, PROHB, DSI, GIT, PDI, PO4HB, P4Hbeta, Prolyl 4-Hydroxylase Subunit Beta, Procollagen-Proline 2-Oxoglutarate 4-Dioxygenase Beta Polypeptide

ID gène: 5034

UniProt: P07237

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism