PRKDC Kit ELISA

N° du produit ABIN5658240

Détail du produit PRKDC Kit ELISA

Antigène: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC). No significant cross-reactivity or interference between Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) and analogues was observed.

Sensibilité: 0.105 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Autre désignation: Protein Kinase, DNA Activated, Catalytic Polypeptide (PRKDC Produits)

Synonymes: DNA-PKcs Kit ELISA, DNAPK Kit ELISA, DNPK1 Kit ELISA, HYRC Kit ELISA, HYRC1 Kit ELISA, XRCC7 Kit ELISA, p350 Kit ELISA, DNA-PKCS Kit ELISA, dna-pkcs Kit ELISA, dnapk Kit ELISA, dnpk1 Kit ELISA, hyrc Kit ELISA, hyrc1 Kit ELISA, prkdc-A Kit ELISA, xrcc7 Kit ELISA, PRKDC Kit ELISA, AI326420 Kit ELISA, AU019811 Kit ELISA, DNAPDcs Kit ELISA, DOXNPH Kit ELISA, dxnph Kit ELISA, p460 Kit ELISA, scid Kit ELISA, slip Kit ELISA, protein kinase, DNA-activated, catalytic polypeptide Kit ELISA, protein kinase, DNA-activated, catalytic polypeptide L homeolog Kit ELISA, DNA-dependent protein kinase catalytic subunit Kit ELISA, protein kinase, DNA activated, catalytic polypeptide Kit ELISA, PRKDC Kit ELISA, prkdc.L Kit ELISA, LOC100633457 Kit ELISA, Prkdc Kit ELISA

Sujet:

Gene Name: Protein Kinase, DNA Activated, Catalytic Polypeptide

Gene Aliases: HYRC, HYRC1, DNPK1, p350, DNAPK, XRCC7, DNA-PKcs

ID gène: 19090

UniProt: P97313

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response