ARHGEF7 Kit ELISA

N° du produit ABIN5658488

Détail du produit ARHGEF7 Kit ELISA

Antigène: ARHGEF7 (rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7). No significant cross-reactivity or interference between Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) and analogues was observed.

Sensibilité: 0.064 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ARHGEF7 (rho Guanine Nucleotide Exchange Factor (GEF) 7 (ARHGEF7))

Autre désignation: Rho Guanine Nucleotide Exchange Factor 7 (ARHGEF7 Produits)

Synonymes: BETA-PIX Kit ELISA, COOL-1 Kit ELISA, COOL1 Kit ELISA, Nbla10314 Kit ELISA, P50 Kit ELISA, P50BP Kit ELISA, P85 Kit ELISA, P85COOL1 Kit ELISA, P85SPR Kit ELISA, PAK3 Kit ELISA, PIXB Kit ELISA, cool1 Kit ELISA, p50 Kit ELISA, p85 Kit ELISA, pak3 Kit ELISA, pixb Kit ELISA, ARHGEF7 Kit ELISA, P85spr Kit ELISA, Pak3bp Kit ELISA, Cool Kit ELISA, PIX Kit ELISA, betaPix Kit ELISA, betaPix-b Kit ELISA, betaPix-c Kit ELISA, cool-1 Kit ELISA, mKIAA0142 Kit ELISA, p85Cool1 Kit ELISA, p85SPR Kit ELISA, arhgef7 Kit ELISA, bbh Kit ELISA, bpix Kit ELISA, im:7142367 Kit ELISA, wu:fc35h08 Kit ELISA, zgc:101775 Kit ELISA, Rho guanine nucleotide exchange factor 7 Kit ELISA, Rho guanine nucleotide exchange factor 7 S homeolog Kit ELISA, ankyrin repeat domain 10 Kit ELISA, Rho guanine nucleotide exchange factor (GEF7) Kit ELISA, Rho guanine nucleotide exchange factor (GEF) 7b Kit ELISA, ARHGEF7 Kit ELISA, arhgef7.S Kit ELISA, arhgef7 Kit ELISA, ANKRD10 Kit ELISA, Arhgef7 Kit ELISA, arhgef7b Kit ELISA

Sujet:

Gene Name: Rho Guanine Nucleotide Exchange Factor 7

Gene Aliases: PAK3, BETA-PIX, COOL1, P50, P50BP, P85, P85SPR, PIXB, SH3 Domain-Containing Proline-Rich Protein, PAK-Interacting Exchange Factor Beta

ID gène: 114559

UniProt: O55043

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation