SFRP1 Kit ELISA

N° du produit ABIN5658625

Détail du produit SFRP1 Kit ELISA

Antigène: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Secreted Frizzled Related Protein 1 (SFRP1). No significant cross-reactivity or interference between Secreted Frizzled Related Protein 1 (SFRP1) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Frizzled Related Protein 1 (SFRP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secreted Frizzled Related Protein 1 (SFRP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secreted Frizzled Related Protein 1 (SFRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secreted Frizzled Related Protein 1 (SFRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secreted Frizzled Related Protein 1 (SFRP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secreted Frizzled Related Protein 1 (SFRP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Autre désignation: Secreted Frizzled Related Protein 1 (SFRP1 Produits)

Synonymes: FRP Kit ELISA, FRP-1 Kit ELISA, FRP1 Kit ELISA, FrzA Kit ELISA, SARP2 Kit ELISA, 2210415K03Rik Kit ELISA, AW011917 Kit ELISA, AW107218 Kit ELISA, AW742929 Kit ELISA, sFRP-1 Kit ELISA, CSFRP1 Kit ELISA, SFRP1 Kit ELISA, XfrzA Kit ELISA, frp Kit ELISA, frp-1 Kit ELISA, frp1 Kit ELISA, frzA Kit ELISA, sarp2 Kit ELISA, sfrp1 Kit ELISA, LOC100231868 Kit ELISA, Srfp1a Kit ELISA, wu:fd19b09 Kit ELISA, secreted frizzled related protein 1 Kit ELISA, secreted frizzled-related protein 1 Kit ELISA, Secreted frizzled-related protein 1 Kit ELISA, secreted frizzled-related protein 1a Kit ELISA, secreted frizzled-related protein 1b Kit ELISA, SFRP1 Kit ELISA, Sfrp1 Kit ELISA, sfrp1 Kit ELISA, sfrp1a Kit ELISA, sfrp1b Kit ELISA

Sujet:

Gene Name: Secreted Frizzled Related Protein 1

Gene Aliases: FRP1, FRP, FRP-1, FrzA, SARP2, Secreted apoptosis-related protein 2

UniProt: Q9R168

Pathways: Signalisation WNT, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation