Stathmin 1 Kit ELISA

N° du produit ABIN5658942

Détail du produit Stathmin 1 Kit ELISA

Antigène: Stathmin 1 (STMN1)

Reactivité: Humain, Rat, Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Stathmin 1 (STMN1). No significant cross-reactivity or interference between Stathmin 1 (STMN1) and analogues was observed.

Sensibilité: 0.058 ng/M

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stathmin 1 (STMN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stathmin 1 (STMN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stathmin 1 (STMN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Stathmin 1 (STMN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stathmin 1 (STMN1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stathmin 1 (STMN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Stathmin 1 (STMN1)

Autre désignation: Stathmin 1 (STMN1 Produits)

Synonymes: C1orf215 Kit ELISA, LAP18 Kit ELISA, Lag Kit ELISA, OP18 Kit ELISA, PP17 Kit ELISA, PP19 Kit ELISA, PR22 Kit ELISA, SMN Kit ELISA, 19k Kit ELISA, Lap18 Kit ELISA, Op18 Kit ELISA, P18 Kit ELISA, P19 Kit ELISA, Pig Kit ELISA, Pp17 Kit ELISA, Pp18 Kit ELISA, Pp19 Kit ELISA, Pr22 Kit ELISA, Smn Kit ELISA, prosolin Kit ELISA, op18 Kit ELISA, stathmin Kit ELISA, stmn1 Kit ELISA, stmn1a Kit ELISA, lag Kit ELISA, lap18 Kit ELISA, pp17 Kit ELISA, pp19 Kit ELISA, pr22 Kit ELISA, smn Kit ELISA, fj43c10 Kit ELISA, wu:fj38b07 Kit ELISA, wu:fj43c10 Kit ELISA, zgc:110159 Kit ELISA, cb959 Kit ELISA, wu:fb14e04 Kit ELISA, zgc:136942 Kit ELISA, stmn1-a Kit ELISA, stmn1b Kit ELISA, xo35 Kit ELISA, stathmin 1 Kit ELISA, stathmin 1 S homeolog Kit ELISA, stathmin 1b Kit ELISA, stathmin 1a Kit ELISA, stathmin-like Kit ELISA, stathmin Kit ELISA, stathmin 1 L homeolog Kit ELISA, STMN1 Kit ELISA, Stmn1 Kit ELISA, stmn1.S Kit ELISA, stmn1 Kit ELISA, stmn1b Kit ELISA, stmn1a Kit ELISA, LOC100229194 Kit ELISA, stmn1.L Kit ELISA

Sujet:

Gene Name: Stathmin 1

Gene Aliases: LAP18, C1orf215, Lag, OP18, PP17, PP19, PR22, SMN, Metablastin, Prosolin, Oncoprotein 18, Leukemia-associated phosphoprotein p18

Pathways: Signalisation MAPK, Dynamique des Microtubules