CXCL12 Kit ELISA

N° du produit ABIN5658968

Détail du produit CXCL12 Kit ELISA

Antigène: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Stromal Cell Derived Factor 1 (SDF1). No significant cross-reactivity or interference between Stromal Cell Derived Factor 1 (SDF1) and analogues was observed.

Sensibilité: 23.3 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stromal Cell Derived Factor 1 (SDF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stromal Cell Derived Factor 1 (SDF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stromal Cell Derived Factor 1 (SDF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Stromal Cell Derived Factor 1 (SDF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stromal Cell Derived Factor 1 (SDF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stromal Cell Derived Factor 1 (SDF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Autre désignation: Stromal Cell Derived Factor 1 (CXCL12 Produits)

Synonymes: IRH Kit ELISA, PBSF Kit ELISA, SCYB12 Kit ELISA, SDF1 Kit ELISA, TLSF Kit ELISA, TPAR1 Kit ELISA, CINC-1 Kit ELISA, Gro1 Kit ELISA, Pbsf Kit ELISA, Scyb12 Kit ELISA, Sdf1 Kit ELISA, Tlsf Kit ELISA, Tpar1 Kit ELISA, Fsp Kit ELISA, KC Kit ELISA, Mgsa Kit ELISA, N51 Kit ELISA, Scyb1 Kit ELISA, gro Kit ELISA, sdf-1 Kit ELISA, sdf1 Kit ELISA, xSDF-1 Kit ELISA, SDF-1 Kit ELISA, cxcl12 Kit ELISA, sdf-1a Kit ELISA, sdf1a Kit ELISA, wu:fa55e10 Kit ELISA, wu:fc16h12 Kit ELISA, wu:fj84c02 Kit ELISA, C-X-C motif chemokine ligand 12 Kit ELISA, C-X-C motif chemokine ligand 1 Kit ELISA, chemokine (C-X-C motif) ligand 12 Kit ELISA, chemokine (C-X-C motif) ligand 1 Kit ELISA, C-X-C motif chemokine ligand 12 L homeolog Kit ELISA, chemokine (C-X-C motif) ligand 12a (stromal cell-derived factor 1) Kit ELISA, CXCL12 Kit ELISA, cxcl12 Kit ELISA, Cxcl1 Kit ELISA, Cxcl12 Kit ELISA, cxcl12.L Kit ELISA, cxcl12a Kit ELISA

Sujet:

Gene Name: Stromal Cell Derived Factor 1

Gene Aliases: CXCL12, PBSF, P-BSF, SCYB12, SDF1-A, SDF1-B, SDF1A, SDF1B, TLSF-A, TLSF-B, TPAR1, Pre-B Cell Growth-Stimulating Factor, Chemokine C-X-C-Motif Ligand 12

ID gène: 20315

UniProt: P40224

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling