SOCS3 Kit ELISA

N° du produit ABIN5659021

Détail du produit SOCS3 Kit ELISA

Antigène: SOCS3 (Suppressor of Cytokine Signaling 3 (SOCS3))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Suppressors Of Cytokine Signaling 3 (SOCS3). No significant cross-reactivity or interference between Suppressors Of Cytokine Signaling 3 (SOCS3) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Suppressors Of Cytokine Signaling 3 (SOCS3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Suppressors Of Cytokine Signaling 3 (SOCS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Suppressors Of Cytokine Signaling 3 (SOCS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Suppressors Of Cytokine Signaling 3 (SOCS3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Suppressors Of Cytokine Signaling 3 (SOCS3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Suppressors Of Cytokine Signaling 3 (SOCS3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SOCS3 (Suppressor of Cytokine Signaling 3 (SOCS3))

Autre désignation: Suppressors Of Cytokine Signaling 3 (SOCS3 Produits)

Synonymes: socs3 Kit ELISA, fj09e12 Kit ELISA, zgc:56537 Kit ELISA, wu:fj09e12 Kit ELISA, MGC64393 Kit ELISA, MGC75749 Kit ELISA, xsocs3 Kit ELISA, MGC81248 Kit ELISA, atod4 Kit ELISA, cis3 Kit ELISA, cish3 Kit ELISA, socs-3 Kit ELISA, ssi-3 Kit ELISA, ssi3 Kit ELISA, SOCS3 Kit ELISA, ATOD4 Kit ELISA, CIS3 Kit ELISA, Cish3 Kit ELISA, SOCS-3 Kit ELISA, SSI-3 Kit ELISA, SSI3 Kit ELISA, Cis3 Kit ELISA, EF-10 Kit ELISA, Ef10 Kit ELISA, Ssi3 Kit ELISA, Socs-3 Kit ELISA, Ssi-3 Kit ELISA, suppressor of cytokine signaling 3a Kit ELISA, suppressor of cytokine signaling 3 S homeolog Kit ELISA, suppressor of cytokine signaling 3 Kit ELISA, suppressor of cytokine signaling 3 L homeolog Kit ELISA, socs3a Kit ELISA, socs3.S Kit ELISA, socs3 Kit ELISA, socs3.L Kit ELISA, SOCS3 Kit ELISA, Socs3 Kit ELISA

Sujet:

Gene Name: Suppressors Of Cytokine Signaling 3

Gene Aliases: ATOD4, CIS3, Cish3, MGC71791, SOCS-3, SSI-3, SSI3, Cytokine-inducible SH2 protein 3, STAT-induced STAT inhibitor 3

ID gène: 89829

UniProt: O88583

Pathways: Signalistation JAK/STAT, Response to Growth Hormone Stimulus, Hepatitis C