TRF1 Kit ELISA

N° du produit ABIN5659111

Le kit ELISA TRF1 (ABIN5659111) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain TRF1.

Aperçu rapide pour TRF1 Kit ELISA (ABIN5659111)

Antigène: TRF1 (TERF1) (Telomeric Repeat Binding Factor (NIMA-Interacting) 1 (TERF1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Détail du produit TRF1 Kit ELISA

Seuil minimal de détection: 0.156 ng/mL

Fonction: Human Telomeric Repeat Binding Factor 1 (TERF1) ELISA Kit for detecting TERF1 in tissue homogenates, cell lysates

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Telomeric Repeat Binding Factor 1 (TERF1). No significant cross-reactivity or interference between Telomeric Repeat Binding Factor 1 (TERF1) and analogues was observed.

Sensibilité: 0.055 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standar
• Detection Reagent A
• Detection Reagent B
• TMB Substrate
• Stop Solution
• Assay Diluent A
• Assay Diluent B
• Wash Buffer (30x concentrate)
• Standard Diluent
• Plate Sealer
• Instruction Manual

Matériel non inclus:

• Microplate Reader with 450 nm filter
• Single or multi-channel pipettes with high precision and disposable tips
• Microcentrifuge tubes
• Deionized or distilled watter
• Absorbent paper for blotting the microplate
• Container for wash solution
• 0.01 M (or 1x) Phosphate Buffered Saline (PBS), ph 7.0-7.2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

This product is tested and assembled before shipping to ensure that it meets its quality specifications. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Telomeric Repeat Binding Factor 1 (TERF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Telomeric Repeat Binding Factor 1 (TERF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Telomeric Repeat Binding Factor 1 (TERF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Telomeric Repeat Binding Factor 1 (TERF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Telomeric Repeat Binding Factor 1 (TERF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Telomeric Repeat Binding Factor 1 (TERF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: TRF1 (TERF1) (Telomeric Repeat Binding Factor (NIMA-Interacting) 1 (TERF1))

Autre désignation: Telomeric Repeat Binding Factor 1

Sujet: Gene Aliases: TRF, PIN2, TRBF1, TRF1, hTRF1-AS, t-TRF1, NIMA-interacting protein 2, TTAGGG repeat-binding factor 1, Telomeric protein Pin2/TRF1

ID gène: 7013

UniProt: P54274

Pathways: Cycle Cellulaire, Telomere Maintenance, Hormone Transport, Chromatin Binding, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity