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TRF1 Kit ELISA

TERF1 Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5659111
  • Antigène Voir toutes TRF1 (TERF1) Kits ELISA
    TRF1 (TERF1) (Telomeric Repeat Binding Factor (NIMA-Interacting) 1 (TERF1))
    Reactivité
    • 4
    • 2
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Telomeric Repeat Binding Factor 1 (TERF1). No significant cross-reactivity or interference between Telomeric Repeat Binding Factor 1 (TERF1) and analogues was observed.
    Sensibilité
    0.055 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Telomeric Repeat Binding Factor 1 (TERF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Telomeric Repeat Binding Factor 1 (TERF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Telomeric Repeat Binding Factor 1 (TERF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Telomeric Repeat Binding Factor 1 (TERF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Telomeric Repeat Binding Factor 1 (TERF1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Telomeric Repeat Binding Factor 1 (TERF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes TRF1 (TERF1) Kits ELISA
    TRF1 (TERF1) (Telomeric Repeat Binding Factor (NIMA-Interacting) 1 (TERF1))
    Autre désignation
    Telomeric Repeat Binding Factor 1 (TERF1 Produits)
    Synonymes
    PIN2 Kit ELISA, TRBF1 Kit ELISA, TRF Kit ELISA, TRF1 Kit ELISA, hTRF1-AS Kit ELISA, t-TRF1 Kit ELISA, wu:fb74c04 Kit ELISA, TERF1 Kit ELISA, DKFZp459A0713 Kit ELISA, Pin2 Kit ELISA, Trbf1 Kit ELISA, Trf1 Kit ELISA, terf1 Kit ELISA, trf1 Kit ELISA, telomeric repeat binding factor 1 Kit ELISA, telomeric repeat binding factor (NIMA-interacting) 1 Kit ELISA, telomeric repeat binding factor (NIMA-interacting) 1 L homeolog Kit ELISA, TERF1 Kit ELISA, Terf1 Kit ELISA, terf1 Kit ELISA, Trf1 Kit ELISA, terf1.L Kit ELISA
    Sujet

    Gene Name: Telomeric Repeat Binding Factor 1

    Gene Aliases: TRF, PIN2, TRBF1, TRF1, hTRF1-AS, t-TRF1, NIMA-interacting protein 2, TTAGGG repeat-binding factor 1, Telomeric protein Pin2/TRF1

    ID gène
    7013
    UniProt
    P54274
    Pathways
    Cycle Cellulaire, Telomere Maintenance, Hormone Transport, Chromatin Binding, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity
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