TXN Kit CLIA

N° du produit ABIN5659146

Détail du produit TXN Kit CLIA

Antigène: TXN (Thioredoxin (TXN))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Thioredoxin (Trx). No significant cross-reactivity or interference between Thioredoxin (Trx) and analogues was observed.

Sensibilité: 2.53 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Thioredoxin (Trx). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Thioredoxin (Trx). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Thioredoxin (Trx) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thioredoxin (Trx) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thioredoxin (Trx) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TXN (Thioredoxin (TXN))

Autre désignation: Thioredoxin (TXN Produits)

Synonymes: TRDX Kit CLIA, TRX Kit CLIA, TRX1 Kit CLIA, ADF Kit CLIA, AW550880 Kit CLIA, Trx1 Kit CLIA, Txn Kit CLIA, trx Kit CLIA, TXN Kit CLIA, SCM1.18 Kit CLIA, trxA3 Kit CLIA, thioredoxin Kit CLIA, TR-1 Kit CLIA, Trx-1 Kit CLIA, thioredoxin Kit CLIA, thioredoxin 1 Kit CLIA, thioredoxin L homeolog Kit CLIA, similar to 44RRORF109c hypothetical protein; similar to bacterial thioredoxin (SwissProt P06544) Kit CLIA, thioredoxin pseudogene Kit CLIA, cytosolic thioredoxin Trx1 Kit CLIA, TXN Kit CLIA, Txn1 Kit CLIA, txn.L Kit CLIA, txn Kit CLIA, trx Kit CLIA, SCO0885 Kit CLIA, PHG25ORF099c Kit CLIA, LOC100349046 Kit CLIA, Txn Kit CLIA, trx1 Kit CLIA

Sujet:

Gene Name: Thioredoxin

Gene Aliases: TXN, ADF, SASP, TRDX, TRX1, ATL-derived factor, Surface-associated sulphydryl protein

ID gène: 7295

UniProt: P10599

Pathways: Carbohydrate Homeostasis, Cell RedoxHomeostasis