TXNIP Kit ELISA

N° du produit ABIN5659153

Détail du produit TXNIP Kit ELISA

Antigène: TXNIP (Thioredoxin Interacting Protein (TXNIP))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Thioredoxin Binding Protein 2 (TBP2). No significant cross-reactivity or interference between Thioredoxin Binding Protein 2 (TBP2) and analogues was observed.

Sensibilité: 0.114 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thioredoxin Binding Protein 2 (TBP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thioredoxin Binding Protein 2 (TBP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thioredoxin Binding Protein 2 (TBP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thioredoxin Binding Protein 2 (TBP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thioredoxin Binding Protein 2 (TBP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thioredoxin Binding Protein 2 (TBP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TXNIP (Thioredoxin Interacting Protein (TXNIP))

Autre désignation: Thioredoxin Binding Protein 2 (TXNIP Produits)

Synonymes: EST01027 Kit ELISA, HHCPA78 Kit ELISA, THIF Kit ELISA, VDUP1 Kit ELISA, Gm348 Kit ELISA, Trf3 Kit ELISA, TBP2 Kit ELISA, TRF3 Kit ELISA, 1200008J08Rik Kit ELISA, AA682105 Kit ELISA, Hyplip1 Kit ELISA, Tbp-2 Kit ELISA, Vdup1 Kit ELISA, sb:cb368 Kit ELISA, txnip Kit ELISA, thioredoxin interacting protein Kit ELISA, TATA box binding protein like 2 Kit ELISA, TATA-box binding protein like 2 Kit ELISA, thioredoxin interacting protein L homeolog Kit ELISA, thioredoxin interacting protein a Kit ELISA, TXNIP Kit ELISA, Tbpl2 Kit ELISA, TBPL2 Kit ELISA, Txnip Kit ELISA, txnip.L Kit ELISA, txnip Kit ELISA, txnipa Kit ELISA

Sujet:

Gene Name: Thioredoxin Binding Protein 2

Gene Aliases: TXNIP, HHCPA78, THIF, VDUP1, Thioredoxin Interacting Protein, Vitamin D3-Upregulated Protein 1, Upregulated By 1,25-Dihydroxyvitamin D-3

ID gène: 56338

UniProt: Q8BG60

Pathways: Protein targeting to Nucleus, Platelet-derived growth Factor Receptor Signaling, Inflammasome