TGFB1 Kit ELISA

N° du produit ABIN5659433

Détail du produit TGFB1 Kit ELISA

Antigène: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivité: Porc

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Platelet-Poor Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 1 (TGFb1). No significant cross-reactivity or interference between Transforming Growth Factor Beta 1 (TGFb1) and analogues was observed.

Sensibilité: 11.9 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta 1 (TGFb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transforming Growth Factor Beta 1 (TGFb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Autre désignation: Transforming Growth Factor Beta 1 (TGFB1 Produits)

Synonymes: CED Kit ELISA, DPD1 Kit ELISA, LAP Kit ELISA, TGFB Kit ELISA, TGFbeta Kit ELISA, TGF-beta Kit ELISA, TGF-BETA-1 Kit ELISA, TGF-beta5 Kit ELISA, ced Kit ELISA, dpd1 Kit ELISA, lap Kit ELISA, tgf-beta Kit ELISA, tgfb Kit ELISA, tgfb5 Kit ELISA, tgfbeta Kit ELISA, TGF-beta1 Kit ELISA, TGFbeta1 Kit ELISA, Tgfb Kit ELISA, Tgfb-1 Kit ELISA, ai39657 Kit ELISA, tgfb1 Kit ELISA, wu:fb13a07 Kit ELISA, xx:ai39657 Kit ELISA, TGFB1 Kit ELISA, csd Kit ELISA, cdb1 Kit ELISA, cdg2 Kit ELISA, csd1 Kit ELISA, csd2 Kit ELISA, csd3 Kit ELISA, ebmd Kit ELISA, lcd1 Kit ELISA, bigh3 Kit ELISA, cdgg1 Kit ELISA, betaig-h3 Kit ELISA, TGFB4 Kit ELISA, transforming growth factor beta 1 Kit ELISA, transforming growth factor beta-1 Kit ELISA, transforming growth factor beta 1 L homeolog Kit ELISA, transforming growth factor, beta 1 Kit ELISA, transforming growth factor, beta 1a Kit ELISA, transforming growth factor beta induced L homeolog Kit ELISA, TGFB1 Kit ELISA, Tgfb1 Kit ELISA, tgfb1.L Kit ELISA, tgfb1a Kit ELISA, tgfbi.L Kit ELISA

Sujet:

Gene Name: Transforming Growth Factor Beta 1

Gene Aliases: TGF-B1, CED, DPD1, LAP, Camurati-Engelmann Disease, Latency-associated peptide

ID gène: 397078

UniProt: P07200

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints