14-3-3 zeta Kit ELISA

N° du produit ABIN5659710

Détail du produit 14-3-3 zeta Kit ELISA

Antigène: 14-3-3 zeta (YWHAZ)

Reactivité: Humain, Rat, Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz). No significant cross-reactivity or interference between Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) and analogues was observed.

Sensibilité: 0.116 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: 14-3-3 zeta (YWHAZ)

Autre désignation: Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAZ Produits)

Synonymes: 14-3-3-zeta Kit ELISA, KCIP-1 Kit ELISA, YWHAD Kit ELISA, 14-3-3zeta Kit ELISA, Ywhaz Kit ELISA, ACYPI003154 Kit ELISA, 14-3-3z Kit ELISA, kcip-1 Kit ELISA, ywhaq Kit ELISA, 1433z Kit ELISA, ywhaz Kit ELISA, ywhazb Kit ELISA, 1110013I11Rik Kit ELISA, AI596267 Kit ELISA, AL022924 Kit ELISA, AU020854 Kit ELISA, ywhaza Kit ELISA, fb14h09 Kit ELISA, wu:fb05g08 Kit ELISA, wu:fb14h09 Kit ELISA, ywhai Kit ELISA, zgc:55807 Kit ELISA, 14-3-3 Kit ELISA, 14-3-3 zeta Kit ELISA, 14-3-3ZETA Kit ELISA, 14-3-3leo Kit ELISA, 2G1 Kit ELISA, 4-3-3 zeta Kit ELISA, 5.11 Kit ELISA, 549 Kit ELISA, BEST:GH05075 Kit ELISA, CG17870 Kit ELISA, D14-3-3 Kit ELISA, D14-3-3zeta Kit ELISA, Dmel\\CG17870 Kit ELISA, K Kit ELISA, LEO Kit ELISA, Leo Kit ELISA, PAR-5 Kit ELISA, PAR5 Kit ELISA, Par-5 Kit ELISA, d14-3-3zeta Kit ELISA, l(2)07103 Kit ELISA, l(2)46CFe Kit ELISA, l(2)46Ee Kit ELISA, leo Kit ELISA, par-5 Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta Kit ELISA, 14-3-3 protein zeta Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta L homeolog Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta S homeolog Kit ELISA, 14-3-3 protein zeta/delta pseudogene Kit ELISA, CG17870 gene product from transcript CG17870-RE Kit ELISA, YWHAZ Kit ELISA, 14-3-3zeta Kit ELISA, ywhaz Kit ELISA, 1433z Kit ELISA, ywhaz.L Kit ELISA, Ywhaz Kit ELISA, ywhaz.S Kit ELISA, LOC100855903 Kit ELISA

Sujet:

Gene Name: Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta

Gene Aliases: KCIP-1, 14-3-3-zeta, 14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1

Pathways: Apoptose, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location