WISP1 Kit CLIA

N° du produit ABIN5660035

Détail du produit WISP1 Kit CLIA

Antigène: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Reactivité: Souris

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of WNT1 Inducible Signaling Pathway Protein 1 (WISP1). No significant cross-reactivity or interference between WNT1 Inducible Signaling Pathway Protein 1 (WISP1) and analogues was observed.

Sensibilité: 1.21 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to WNT1 Inducible Signaling Pathway Protein 1 (WISP1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to WNT1 Inducible Signaling Pathway Protein 1 (WISP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the WNT1 Inducible Signaling Pathway Protein 1 (WISP1) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WNT1 Inducible Signaling Pathway Protein 1 (WISP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WNT1 Inducible Signaling Pathway Protein 1 (WISP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Autre désignation: WNT1 Inducible Signaling Pathway Protein 1 (WISP1 Produits)

Synonymes: CCN4 Kit CLIA, WISP1c Kit CLIA, WISP1i Kit CLIA, WISP1tc Kit CLIA, WISP-1 Kit CLIA, WISP1 Kit CLIA, si:ch211-38f10.2 Kit CLIA, AW146261 Kit CLIA, Elm1 Kit CLIA, zgc:172276 Kit CLIA, WNT1 inducible signaling pathway protein 1 Kit CLIA, WNT1 inducible signaling pathway protein 1a Kit CLIA, WNT1-inducible-signaling pathway protein 1-like Kit CLIA, WNT1 inducible signaling pathway protein 1b Kit CLIA, WISP1 Kit CLIA, wisp1a Kit CLIA, Wisp1 Kit CLIA, LOC100627044 Kit CLIA, wisp1b Kit CLIA

Sujet:

Gene Name: WNT1 Inducible Signaling Pathway Protein 1

Gene Aliases: CCN4, WISP1c, WISP1i, WISP1tc, CCN family member 4, Wnt-1-induced secreted protein

ID gène: 22402

UniProt: O54775

Pathways: Signalisation WNT, Growth Factor Binding