WISP1 Kit ELISA

N° du produit ABIN5660036

Détail du produit WISP1 Kit ELISA

Antigène: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 6.25 pg/mL - 400 pg/mL

Seuil minimal de détection: 6.25 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of WNT1 Inducible Signaling Pathway Protein 1 (WISP1). No significant cross-reactivity or interference between WNT1 Inducible Signaling Pathway Protein 1 (WISP1) and analogues was observed.

Sensibilité: 2.55 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT1 Inducible Signaling Pathway Protein 1 (WISP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WNT1 Inducible Signaling Pathway Protein 1 (WISP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT1 Inducible Signaling Pathway Protein 1 (WISP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of WNT1 Inducible Signaling Pathway Protein 1 (WISP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WNT1 Inducible Signaling Pathway Protein 1 (WISP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WNT1 Inducible Signaling Pathway Protein 1 (WISP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Autre désignation: WNT1 Inducible Signaling Pathway Protein 1 (WISP1 Produits)

Synonymes: CCN4 Kit ELISA, WISP1c Kit ELISA, WISP1i Kit ELISA, WISP1tc Kit ELISA, WISP-1 Kit ELISA, WISP1 Kit ELISA, si:ch211-38f10.2 Kit ELISA, AW146261 Kit ELISA, Elm1 Kit ELISA, zgc:172276 Kit ELISA, WNT1 inducible signaling pathway protein 1 Kit ELISA, WNT1 inducible signaling pathway protein 1a Kit ELISA, WNT1-inducible-signaling pathway protein 1-like Kit ELISA, WNT1 inducible signaling pathway protein 1b Kit ELISA, WISP1 Kit ELISA, wisp1a Kit ELISA, Wisp1 Kit ELISA, LOC100627044 Kit ELISA, wisp1b Kit ELISA

Sujet:

Gene Name: WNT1 Inducible Signaling Pathway Protein 1

Gene Aliases: CCN4, WISP1c, WISP1i, WISP1tc, CCN family member 4, Wnt-1-induced secreted protein

ID gène: 22402

UniProt: O54775

Pathways: Signalisation WNT, Growth Factor Binding