YAP1 Kit ELISA
-
- Antigène Voir toutes YAP1 Kits ELISA
- YAP1 (Yes-Associated Protein 1 (YAP1))
-
Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.156 ng/mL - 10 ng/mL
- Seuil minimal de détection
- 0.156 ng/mL
- Application
- ELISA
- Type d'échantillon
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Yes Associated Protein 1 (YAP1). No significant cross-reactivity or interference between Yes Associated Protein 1 (YAP1) and analogues was observed.
- Sensibilité
- 0.056 ng/mL
- Featured
- Discover our best selling YAP1 Kit ELISA
- Top Product
- Discover our top product YAP1 Kit ELISA
-
-
- Commentaires
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Yes Associated Protein 1 (YAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Yes Associated Protein 1 (YAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Yes Associated Protein 1 (YAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Yes Associated Protein 1 (YAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Yes Associated Protein 1 (YAP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Yes Associated Protein 1 (YAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
-
- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
-
- Antigène Voir toutes YAP1 Kits ELISA
- YAP1 (Yes-Associated Protein 1 (YAP1))
- Autre désignation
- Yes Associated Protein 1 (YAP1 Produits)
- Synonymes
- YAP Kit ELISA, YAP2 Kit ELISA, YAP65 Kit ELISA, YKI Kit ELISA, xyap Kit ELISA, yap Kit ELISA, yap2 Kit ELISA, yap65 Kit ELISA, yki Kit ELISA, AI325207 Kit ELISA, Yap Kit ELISA, Yap65 Kit ELISA, Yki Kit ELISA, Yorkie Kit ELISA, cb194 Kit ELISA, sb:cb194 Kit ELISA, si:ch211-181p1.5 Kit ELISA, si:dkey-3b8.3 Kit ELISA, wu:fc18c04 Kit ELISA, zgc:158380 Kit ELISA, Yes associated protein 1 Kit ELISA, Yes associated protein 1 S homeolog Kit ELISA, yes-associated protein 1 Kit ELISA, Yes-associated protein 1 Kit ELISA, YAP1 Kit ELISA, yap1.S Kit ELISA, Yap1 Kit ELISA, yap1 Kit ELISA
- Sujet
-
Gene Name: Yes Associated Protein 1
Gene Aliases: YAP, YAP2, YAP65, Transcriptional coactivator YAP1, Protein yorkie homolog, Yes-associated protein YAP65 homolog
- Pathways
- Signalisation MAPK, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
-