ADORA1 Kit ELISA

N° du produit ABIN5674664

Détail du produit ADORA1 Kit ELISA

Antigène: ADORA1 (Adenosine A1 Receptor (ADORA1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0061 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: ADORA1 (Adenosine A1 Receptor (ADORA1))

Abstract: ADORA1 Produits

Synonymes: adora1-A Kit ELISA, a1R Kit ELISA, ADORA1 Kit ELISA, LOC606743 Kit ELISA, LOC100222054 Kit ELISA, RDC7 Kit ELISA, A1-AR Kit ELISA, A1AR Kit ELISA, A1R Kit ELISA, AA1R Kit ELISA, AI848715 Kit ELISA, BB176431 Kit ELISA, Ri Kit ELISA, adenosine A1 receptor L homeolog Kit ELISA, adenosine A1 receptor Kit ELISA, adenosine receptor A1 Kit ELISA, adora1.L Kit ELISA, ADORA1 Kit ELISA, adora1 Kit ELISA, LOC100222054 Kit ELISA, Adora1 Kit ELISA

Sujet: Alternative name: RDC7

ID gène: 134

UniProt: P30542

Pathways: EGFR Signaling Pathway, Negative Regulation of Hormone Secretion, Synaptic Membrane