C9 Kit ELISA

N° du produit ABIN5674670

Détail du produit C9 Kit ELISA

Antigène: C9 (Complement Component C9 (C9))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.468 ng/mL - 30 ng/mL

Seuil minimal de détection: 0.468 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0.19 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: C9 (Complement Component C9 (C9))

Autre désignation: Complement Component 9 (C9) (C9 Produits)

Synonymes: LOC100037951 Kit ELISA, LOC100136130 Kit ELISA, c9 Kit ELISA, wu:fb60b05 Kit ELISA, wu:fd50e04 Kit ELISA, zgc:112272 Kit ELISA, complement C9 Kit ELISA, complement component 9 Kit ELISA, complement component 9 L homeolog Kit ELISA, C9 Kit ELISA, c9 Kit ELISA, c9.L Kit ELISA

Sujet: Alternative name: Complement component C9, C9

ID gène: 12279

UniProt: P06683

Pathways: Système du Complément