SOCS2 Kit ELISA

N° du produit ABIN5674723

Détail du produit SOCS2 Kit ELISA

Antigène: SOCS2 (Suppressor of Cytokine Signaling 2 (SOCS2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0059 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: SOCS2 (Suppressor of Cytokine Signaling 2 (SOCS2))

Autre désignation: Suppressors Of Cytokine Signaling 2 (SOCS2) (SOCS2 Produits)

Synonymes: CIS2 Kit ELISA, Cish2 Kit ELISA, SOCS-2 Kit ELISA, SSI-2 Kit ELISA, SSI2 Kit ELISA, STATI2 Kit ELISA, Cish1 Kit ELISA, Socs-1 Kit ELISA, Socs-2 Kit ELISA, si:dkey-202b22.1 Kit ELISA, socs2 Kit ELISA, cis2 Kit ELISA, ssi2 Kit ELISA, cish2 Kit ELISA, ssi-2 Kit ELISA, socs-2 Kit ELISA, stati2 Kit ELISA, 8030460M17 Kit ELISA, AI527257 Kit ELISA, AW108012 Kit ELISA, D130043N08Rik Kit ELISA, JAB Kit ELISA, hg Kit ELISA, Cradd Kit ELISA, Socs2 Kit ELISA, suppressor of cytokine signaling 2 Kit ELISA, suppressor of cytokine signaling 1 Kit ELISA, death domain-containing protein CRADD Kit ELISA, suppressor of cytokine signaling 2 S homeolog Kit ELISA, SOCS2 Kit ELISA, Socs1 Kit ELISA, Socs2 Kit ELISA, socs2 Kit ELISA, LOC100716104 Kit ELISA, socs2.S Kit ELISA

Sujet: Alternative name: CIS2, Cish2, SOCS-2, SSI-2, SSI2, STATI2, Cytokine-inducible SH2 protein 2, STAT-induced STAT inhibitor 2

ID gène: 8835

UniProt: O14508

Pathways: Signalistation JAK/STAT, Response to Growth Hormone Stimulus