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ADCY10 Kit ELISA

ADCY10 Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
N° du produit ABIN5674728
  • Antigène Voir toutes ADCY10 Kits ELISA
    ADCY10 (Adenylate Cyclase 10 (Soluble) (ADCY10))
    Reactivité
    • 2
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    0051 ng/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes ADCY10 Kits ELISA
    ADCY10 (Adenylate Cyclase 10 (Soluble) (ADCY10))
    Autre désignation
    Adenylate Cyclase 10, Soluble (ADCY10) (ADCY10 Produits)
    Synonymes
    HCA2 Kit ELISA, RP1-313L4.2 Kit ELISA, SAC Kit ELISA, SACI Kit ELISA, Sacy Kit ELISA, Sac Kit ELISA, 4930431D04Rik Kit ELISA, 4931412F17 Kit ELISA, sAC Kit ELISA, ADCY10 Kit ELISA, SACY Kit ELISA, adenylate cyclase 10 Kit ELISA, adenylate cyclase 10 (soluble) Kit ELISA, ADCY10 Kit ELISA, Adcy10 Kit ELISA
    Sujet
    Alternative name: SAC, HCA2, SACI, Sacy, Testicular soluble adenylyl cyclase, Hypercalciuria,Absorptive 2, AH-related protein, Germ cell soluble adenylyl cyclase
    ID gène
    55811
    UniProt
    Q96PN6
    Pathways
    cAMP Metabolic Process
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