SIRT2 Kit ELISA

N° du produit ABIN5674759

Détail du produit SIRT2 Kit ELISA

Antigène: SIRT2 (Sirtuin 2 (SIRT2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0.61 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: SIRT2 (Sirtuin 2 (SIRT2))

Abstract: SIRT2 Produits

Synonymes: CG5085 Kit ELISA, D.mel2 Kit ELISA, Dmel\\CG5085 Kit ELISA, SIRT2 Kit ELISA, dSIRT2 Kit ELISA, sir2 Kit ELISA, Sirt2 Kit ELISA, BmSirt2 Kit ELISA, AtSRT2 Kit ELISA, SIRTUIN 2 Kit ELISA, T2K12.8 Kit ELISA, sirtuin 2 Kit ELISA, SIR2 Kit ELISA, SIR2L Kit ELISA, SIR2L2 Kit ELISA, 5730427M03Rik Kit ELISA, Sir2l Kit ELISA, wu:fb57d05 Kit ELISA, zgc:55966 Kit ELISA, zgc:77003 Kit ELISA, Sirtuin 2 Kit ELISA, sirtuin 2 Kit ELISA, sirtuin 2 S homeolog Kit ELISA, NAD-dependent deacetylase sirtuin 2 homolog Kit ELISA, sirtuin 2 (silent mating type information regulation 2, homolog) 2 (S. cerevisiae) Kit ELISA, Sirt2 Kit ELISA, SIRT2 Kit ELISA, sirt2.S Kit ELISA, SRT2 Kit ELISA, sirt2 Kit ELISA

Sujet: Alternative name: SIR2L, SIR2-L, SIR2L2, Silent Information Regulator 2 Protein, NAD-Dependent Deacetylase Sirtuin-2, Silent Mating Type Information Regulation 2 Homolog

ID gène: 64383

UniProt: Q8VDQ8