NME1 Kit ELISA

N° du produit ABIN5674913

Détail du produit NME1 Kit ELISA

Antigène: NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Sensibilité: 0059 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))

Autre désignation: Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) (NME1 Produits)

Synonymes: AWD Kit ELISA, GAAD Kit ELISA, NB Kit ELISA, NBS Kit ELISA, NDKA Kit ELISA, NDPK-A Kit ELISA, NDPKA Kit ELISA, NM23 Kit ELISA, NM23-H1 Kit ELISA, AL024257 Kit ELISA, NM23-M1 Kit ELISA, NM23A Kit ELISA, NDPK-Z1 Kit ELISA, NM23-B Kit ELISA, NM23-Z1 Kit ELISA, ndpkz1 Kit ELISA, nm23b Kit ELISA, nme1 Kit ELISA, nme2 Kit ELISA, nme2b1 Kit ELISA, NME1 Kit ELISA, NME1-NME2 Kit ELISA, NM23-C1 Kit ELISA, ndpk-b Kit ELISA, ndpkb Kit ELISA, nm23-h2 Kit ELISA, nm23ndk Kit ELISA, puf Kit ELISA, NBR-A Kit ELISA, NBR-B Kit ELISA, NME1-1 Kit ELISA, nm23ndk-a Kit ELISA, NDK I Kit ELISA, NDPK I Kit ELISA, T30A10.80 Kit ELISA, T30A10_80 Kit ELISA, NDP kinase I Kit ELISA, NME/NM23 nucleoside diphosphate kinase 1 Kit ELISA, NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 Kit ELISA, non-metastatic cells 1, protein (NM23A) expressed in Kit ELISA, NME/NM23 nucleoside diphosphate kinase 2 S homeolog Kit ELISA, NME/NM23 nucleoside diphosphate kinase 2 L homeolog Kit ELISA, nucleoside diphosphate kinase Kit ELISA, nucleoside diphosphate kinase 1 Kit ELISA, NME1 Kit ELISA, Nme1 Kit ELISA, nme2b.1 Kit ELISA, nme2.S Kit ELISA, nme2.L Kit ELISA, LOC547870 Kit ELISA, NDPK1 Kit ELISA, LOC107790563 Kit ELISA

Sujet: Alternative name: AWD, GAAD, NDPKA, NM23-H1, Nucleoside diphosphate kinase A, Granzyme A-activated DNase, Metastasis inhibition factor nm23, Tumor metastatic process-associated protein

ID gène: 4830

UniProt: P15531

Pathways: Apoptose, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process